The cloning of the cholesterol oxidase gene in several Gram-positive bacteria, including Lactobacillus reuteri of intestinal origin, was obtained. Only the transformants of Escherichia coli harbouring the recombinant plasmid pCHOA showed a good intracellular enzyme activity. The heterologous gene was stably maintained in Gram-positive transformants but no enzyme activity was detected.

Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli / Brigidi, P.; Bolognani, F.; Rossi, Maddalena; Cerr, C.; Matteuzzi, D.. - In: LETTERS IN APPLIED MICROBIOLOGY. - ISSN 0266-8254. - STAMPA. - 17:(1993), pp. 61-64.

Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli

ROSSI, Maddalena;
1993

Abstract

The cloning of the cholesterol oxidase gene in several Gram-positive bacteria, including Lactobacillus reuteri of intestinal origin, was obtained. Only the transformants of Escherichia coli harbouring the recombinant plasmid pCHOA showed a good intracellular enzyme activity. The heterologous gene was stably maintained in Gram-positive transformants but no enzyme activity was detected.
1993
17
61
64
Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli / Brigidi, P.; Bolognani, F.; Rossi, Maddalena; Cerr, C.; Matteuzzi, D.. - In: LETTERS IN APPLIED MICROBIOLOGY. - ISSN 0266-8254. - STAMPA. - 17:(1993), pp. 61-64.
Brigidi, P.; Bolognani, F.; Rossi, Maddalena; Cerr, C.; Matteuzzi, D.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/458088
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