The characterisation of an acellular pertussis vaccine composed of a genetically modified pertussis toxin, filamentous haemagglutinin and pertactin is described. The three antigens are submitted to a mild treatment with formaldehyde in the presence of lysine before their use in vaccine formulation. Characterisation is performed by amino acid analysis, SDS-PAGE, analytical size exclusion chromatography and, in the case of pertactin, isoelectrofocusing. The effect of some variables on pertactin formaldehyde treatment has been studied by means of isoelectrofocusing and mouse immunogenicity.

Physicochemical characterisation of the pertussis vaccine / Ceccarini, C.; Contorni, M.; D'Ascenzi, S.; Gallo, E.; Maffei, M.; Mannucci, D.; Marsili, I.; Magagnoli, C.; Peppoloni, Samuele; Rappuoli, R.; Ravenscroft, N.; Ricci, S.. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - STAMPA. - 103:(2000), pp. 175-188.

Physicochemical characterisation of the pertussis vaccine.

PEPPOLONI, Samuele;
2000

Abstract

The characterisation of an acellular pertussis vaccine composed of a genetically modified pertussis toxin, filamentous haemagglutinin and pertactin is described. The three antigens are submitted to a mild treatment with formaldehyde in the presence of lysine before their use in vaccine formulation. Characterisation is performed by amino acid analysis, SDS-PAGE, analytical size exclusion chromatography and, in the case of pertactin, isoelectrofocusing. The effect of some variables on pertactin formaldehyde treatment has been studied by means of isoelectrofocusing and mouse immunogenicity.
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Physicochemical characterisation of the pertussis vaccine / Ceccarini, C.; Contorni, M.; D'Ascenzi, S.; Gallo, E.; Maffei, M.; Mannucci, D.; Marsili, I.; Magagnoli, C.; Peppoloni, Samuele; Rappuoli, R.; Ravenscroft, N.; Ricci, S.. - In: DEVELOPMENTAL BIOLOGY. - ISSN 0012-1606. - STAMPA. - 103:(2000), pp. 175-188.
Ceccarini, C.; Contorni, M.; D'Ascenzi, S.; Gallo, E.; Maffei, M.; Mannucci, D.; Marsili, I.; Magagnoli, C.; Peppoloni, Samuele; Rappuoli, R.; Ravenscroft, N.; Ricci, S.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/457057
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