The B monomer of the Escherichia coli heat-labile toxin (LTB) was expressed on the surface of the human oral commensal bacterium Streptococcus gordonii. Recombinant bacteria expressing LTB were used to immunize BALB/c mice subcutaneously and intragastrically. The LTB monomer expressed on the streptococcal surface proved to be highly immunogenic, as LTB-specific immunoglobulin G (IgG) serum titers of 140,000 were induced after systemic immunization. Most significantly, these antibodies were capable of neutralizing the enterotoxin in a cell neutralization assay. Following mucosal delivery, antigen-specific IgA antibodies were found in feces and antigen-specific IgG antibodies were found in sera. Analysis of serum IgG subclasses showed a clear predominance of IgG1 when recombinant bacteria were inoculated subcutaneously, while a prevalence of IgG2a was observed upon intragastric delivery, suggesting, in this case, the recruitment of a Th1 type of immune response.

Immunogenicity of the B monomer of Escherichia coli heat-Labile Toxin expressed on the surface of the Streptococcus gordonii / RICCI, S.; MEDAGLINI, B.; RUSH, C.; MARCELLO, A.; PEPPOLONI, Samuele; MANGANELLI, R.; PALU, G.; POZZI, G.. - In: INFECTION AND IMMUNITY. - ISSN 0019-9567. - STAMPA. - 68(2)(2000), pp. 760-766.

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