The simple mucin-type carbohydrate antigens Tn, sialosyl-Tn, T and the 'cryptic' sialylated variant of the last represent the mucin core oligosaccharide structures that are produced in the initial steps of the mucin biosynthetic pathway. Utilizing monoclonal antibodies anti-Tn antigen (HB-Tn1), anti-sialosyl-Tn antigen (HB-STn1), anti-T antigen (HB-T1) and the biotinylated Amaranthus caudatus agglutinin (ACA), we have investigated the expression of the simple mucin-type carbohydrate antigens in hereditary nonpolyposis colorectal cancer (HNPCC, 15 cases) compared with sporadic colorectal cancer (CRC, 60 cases) and normal colonic mucosa (30 cases). A variable positivity of Tn, sialosyl-Tn, T and the cryptic sialylated form of this latter antigen was encountered in both HNPCC and sporadic CRC cases, in addition, in normal colonic mucosa a constant reactivity was encountered only for Tn and the cryptic sialylated form of T, while negative results were always obtained for sialosyl-Tn and T antigens. Statistical analysis, performed using a Chi-square test, showed significantly lower (P = 0.037) expression of sialosyl-Tn and higher (P = 0.022) expression of T in HNPCC than in sporadic CRC, suggesting a greater presence of beta 1, 3 galactosyltransferase activity in HNPCC than in sporadic CRC. We were unable to identify a peculiar phenotype for HNPCC with simultaneous evaluation of reactivity for HB-Tn1, HB-STn1, HB-T1 and ACA, the biological significance of the preferential expression of T antigen in HNPCC remains to be investigated.
|Anno di pubblicazione:||1996|
|Titolo:||Detection of Tn, sialyn-Tn and T antigens in HNPCC|
|Autore/i:||G.GIUFFRÈ; E.VITARELLI; G.TUCCARI; M. PONZ DE LEON PISANI; G.BARRESI|
|Codice identificativo Scopus:||2-s2.0-0029750594|
|Citazione:||Detection of Tn, sialyn-Tn and T antigens in HNPCC / G.GIUFFRÈ; E.VITARELLI; G.TUCCARI; M. PONZ DE LEON PISANI; G.BARRESI. - In: VIRCHOWS ARCHIV. - ISSN 0945-6317. - STAMPA. - 429(1996), pp. 345-352.|
|Tipologia||Articolo su rivista|
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