RAPD analysis was used to determine the genetic similarity in wild species and cultivatedvarieties of Limonium genus and for verification of hybridity in progenies of interspecific crosses.Eleven wild species and ten commercial varieties were tested with ten selected RAPDprimers. A total of 254 bands were scored and used for calculating genetic distances amongentries. Two cultivars were grouped together with L. sinensis. The species L. bellidifolium, L.otolepis and L. caspia were clustered together. The largest cluster grouped five varieties and L.latifolium. The remaining three cultivars, belonging to the ‘Altaica’ group, resulted related toL. gmelinii. The wild species L. tataricum, L. perezii, L. sinuatum and L. longifolium were clearlyseparated from all of the other genotypes.Seven putative interspecific hybrids and their parents were characterised by means of nineselected RAPD primers. The putative parentage was confirmed for only two hybrids; the remainingfive genotypes, on the basis of RAPD patterns, were identified as apomixis and/orself-pollinations.Our results demonstrated that RAPD analysis is a useful tool for the molecular characterizationof Limonium genotypes. This simple technique may enable ornamental plant breedersto improve the efficiency of their breeding programs.
Molecular analysis of Limonium genus through RAPD markers / Bruna, S.; G., Burchi; L., DE BENEDETTI; A., Mercuri; Pecchioni, Nicola; C. BIANCHINI T., Schiva. - In: AGRICOLTURA MEDITERRANEA. - ISSN 0394-0438. - STAMPA. - 135(1):(2005), pp. 52-58.
Molecular analysis of Limonium genus through RAPD markers.
PECCHIONI, Nicola;
2005
Abstract
RAPD analysis was used to determine the genetic similarity in wild species and cultivatedvarieties of Limonium genus and for verification of hybridity in progenies of interspecific crosses.Eleven wild species and ten commercial varieties were tested with ten selected RAPDprimers. A total of 254 bands were scored and used for calculating genetic distances amongentries. Two cultivars were grouped together with L. sinensis. The species L. bellidifolium, L.otolepis and L. caspia were clustered together. The largest cluster grouped five varieties and L.latifolium. The remaining three cultivars, belonging to the ‘Altaica’ group, resulted related toL. gmelinii. The wild species L. tataricum, L. perezii, L. sinuatum and L. longifolium were clearlyseparated from all of the other genotypes.Seven putative interspecific hybrids and their parents were characterised by means of nineselected RAPD primers. The putative parentage was confirmed for only two hybrids; the remainingfive genotypes, on the basis of RAPD patterns, were identified as apomixis and/orself-pollinations.Our results demonstrated that RAPD analysis is a useful tool for the molecular characterizationof Limonium genotypes. This simple technique may enable ornamental plant breedersto improve the efficiency of their breeding programs.
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