Datura arborea and D. sanguinea hairy roots were produced by cocultivation of leaf fragments with Agrobacteriumrhizogenes strain NCPP 1855. Adventitious buds emerged spontaneously-, without exogenous growth regulators, fiom sevenhairy root clones of D. arborea and from one hairy, root clone of D. sanguinea. Regenerated plants were successfullyacclimatized in the greenhouse. The integration of the bacterial TL-DNA into the genome of the putative transformed plantswas confirmed by Southern blot analysis. Transgenie plants displayed increased ability to root in vivo. Morphological traitswith relevant ornamental value like plant height, leaf number, size and shape, internode numbe~, and internode length werealso affected. Transformation by wild-type Ri TL-DNA provided the chance to study plant growth and differentiation andto select improved genotypes.
Characterization of ornamental Datura plants transformed by Agrobacterium rhizogenes / Giovannini, A.; Pecchioni, Nicola; M., Rabaglio; A., Allavena. - In: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT. - ISSN 1054-5476. - STAMPA. - 33:(1997), pp. 101-106.
Characterization of ornamental Datura plants transformed by Agrobacterium rhizogenes.
PECCHIONI, Nicola;
1997
Abstract
Datura arborea and D. sanguinea hairy roots were produced by cocultivation of leaf fragments with Agrobacteriumrhizogenes strain NCPP 1855. Adventitious buds emerged spontaneously-, without exogenous growth regulators, fiom sevenhairy root clones of D. arborea and from one hairy, root clone of D. sanguinea. Regenerated plants were successfullyacclimatized in the greenhouse. The integration of the bacterial TL-DNA into the genome of the putative transformed plantswas confirmed by Southern blot analysis. Transgenie plants displayed increased ability to root in vivo. Morphological traitswith relevant ornamental value like plant height, leaf number, size and shape, internode numbe~, and internode length werealso affected. Transformation by wild-type Ri TL-DNA provided the chance to study plant growth and differentiation andto select improved genotypes.
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