Expressed sequence tags (ESTs) databases are being created for severalplant species. ESTs have the potential to analyse the expressed part ofthe genome, and to be used in a wide range of applications from thetheoretical aspect of plant biology to the breeding process. The presentstudy is based on a cold-stressed cDNA library as a source of ESTs inbarley: partial cDNA sequencing of randomly isolated clones yielded960 ESTs. The strategy of random sequencing cDNA clones at their5' ends allows one to obtain information on the steady-state level of thetranscripts in a cold-stressed green leaf of barley. Repetitive sequencingof nearly identical ESTs provides, in addition, useful information onthe existence of multigene families. The results of a BLAST searchperformed on this set of ESTs showed that 18.5% of the tags exhibitedno significant homology to known genes, 10% had significant levels ofhomology with known cold or other stress-related genes, about 26%proved homologous to enzymes involved in several metabolic pathways and about 15% encoded for ribosomal proteins, cytoskeletonproteins, histones, ubiquitin, elongation factors and others. Redundancy was found for some classes of tag: RuBisCo, cab proteins,carbonic anhydrase, glutamate decarboxylase and stress relatedproteins.

Expressed sequence tags (ESTs) from cold-acclimated barley to identify novel plant genes / Faccioli, P; Pecchioni, Nicola; Cattivelli, L; STANCA A., M; Terzi, V.. - In: PLANT BREEDING. - ISSN 0179-9541. - STAMPA. - 120:(2001), pp. 497-502.

Expressed sequence tags (ESTs) from cold-acclimated barley to identify novel plant genes

PECCHIONI, Nicola;
2001

Abstract

Expressed sequence tags (ESTs) databases are being created for severalplant species. ESTs have the potential to analyse the expressed part ofthe genome, and to be used in a wide range of applications from thetheoretical aspect of plant biology to the breeding process. The presentstudy is based on a cold-stressed cDNA library as a source of ESTs inbarley: partial cDNA sequencing of randomly isolated clones yielded960 ESTs. The strategy of random sequencing cDNA clones at their5' ends allows one to obtain information on the steady-state level of thetranscripts in a cold-stressed green leaf of barley. Repetitive sequencingof nearly identical ESTs provides, in addition, useful information onthe existence of multigene families. The results of a BLAST searchperformed on this set of ESTs showed that 18.5% of the tags exhibitedno significant homology to known genes, 10% had significant levels ofhomology with known cold or other stress-related genes, about 26%proved homologous to enzymes involved in several metabolic pathways and about 15% encoded for ribosomal proteins, cytoskeletonproteins, histones, ubiquitin, elongation factors and others. Redundancy was found for some classes of tag: RuBisCo, cab proteins,carbonic anhydrase, glutamate decarboxylase and stress relatedproteins.
120
497
502
Expressed sequence tags (ESTs) from cold-acclimated barley to identify novel plant genes / Faccioli, P; Pecchioni, Nicola; Cattivelli, L; STANCA A., M; Terzi, V.. - In: PLANT BREEDING. - ISSN 0179-9541. - STAMPA. - 120:(2001), pp. 497-502.
Faccioli, P; Pecchioni, Nicola; Cattivelli, L; STANCA A., M; Terzi, V.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/456221
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