A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein wasisolated from cold-treated barley leaves combining the differential display and the 50-RACE techniques. Cor tmcap3is expressed at low level under normal growing temperature, and its expression is strongly enhanced aftercold treatment. A positive correlation between the expression of cor tmc-ap3 and frost tolerance was found bothamong barley cultivars and among cereal species. The COR TMC-AP3 protein was expressed in vitro, purifiedand used to raise a polyclonal antibody.Western analysis showed that the cor tmc-ap3 gene product is localized tothe chloroplastic outer envelope fraction, supporting its putative function. The frost-resistant winter cultivar Oniceaccumulated COR TMC-AP3 more rapidly and at a higher level than the frost-susceptible spring cultivar Gitane.After 28 days of cold acclimation the winter cultivar had about 2-fold more protein than the spring genotype.All these results suggest that an increased amount of a chloroplastic amino acid selective channel protein couldbe required for cold acclimation in cereals. Hypotheses about the role of COR TMC-AP3 during the hardeningprocess are discussed.
|Anno di pubblicazione:||1999|
|Titolo:||High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals|
|Autore/i:||BALDI P; GROSSI M; N. PECCHIONI; VALÉ G; CATTIVELLI L|
|Codice identificativo ISI:||WOS:000083285500008|
|Codice identificativo Scopus:||2-s2.0-0033197477|
|Citazione:||High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals / BALDI P; GROSSI M; N. PECCHIONI; VALÉ G; CATTIVELLI L. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - STAMPA. - 41(1999), pp. 233-243.|
|Tipologia||Articolo su rivista|
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