A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein wasisolated from cold-treated barley leaves combining the differential display and the 50-RACE techniques. Cor tmcap3is expressed at low level under normal growing temperature, and its expression is strongly enhanced aftercold treatment. A positive correlation between the expression of cor tmc-ap3 and frost tolerance was found bothamong barley cultivars and among cereal species. The COR TMC-AP3 protein was expressed in vitro, purifiedand used to raise a polyclonal antibody.Western analysis showed that the cor tmc-ap3 gene product is localized tothe chloroplastic outer envelope fraction, supporting its putative function. The frost-resistant winter cultivar Oniceaccumulated COR TMC-AP3 more rapidly and at a higher level than the frost-susceptible spring cultivar Gitane.After 28 days of cold acclimation the winter cultivar had about 2-fold more protein than the spring genotype.All these results suggest that an increased amount of a chloroplastic amino acid selective channel protein couldbe required for cold acclimation in cereals. Hypotheses about the role of COR TMC-AP3 during the hardeningprocess are discussed.

High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals / Baldi, P; Grossi, M; Pecchioni, Nicola; Valé, G; Cattivelli, L.. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - STAMPA. - 41:(1999), pp. 233-243.

High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals

PECCHIONI, Nicola;
1999

Abstract

A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein wasisolated from cold-treated barley leaves combining the differential display and the 50-RACE techniques. Cor tmcap3is expressed at low level under normal growing temperature, and its expression is strongly enhanced aftercold treatment. A positive correlation between the expression of cor tmc-ap3 and frost tolerance was found bothamong barley cultivars and among cereal species. The COR TMC-AP3 protein was expressed in vitro, purifiedand used to raise a polyclonal antibody.Western analysis showed that the cor tmc-ap3 gene product is localized tothe chloroplastic outer envelope fraction, supporting its putative function. The frost-resistant winter cultivar Oniceaccumulated COR TMC-AP3 more rapidly and at a higher level than the frost-susceptible spring cultivar Gitane.After 28 days of cold acclimation the winter cultivar had about 2-fold more protein than the spring genotype.All these results suggest that an increased amount of a chloroplastic amino acid selective channel protein couldbe required for cold acclimation in cereals. Hypotheses about the role of COR TMC-AP3 during the hardeningprocess are discussed.
41
233
243
High expression level of a gene coding for a chloroplastic amino acid selective channel protein is correlated to cold acclimation in cereals / Baldi, P; Grossi, M; Pecchioni, Nicola; Valé, G; Cattivelli, L.. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - STAMPA. - 41:(1999), pp. 233-243.
Baldi, P; Grossi, M; Pecchioni, Nicola; Valé, G; Cattivelli, L.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/456219
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 44
  • ???jsp.display-item.citation.isi??? 42
social impact