A major gene underlying quantitative resistance to barley leaf stripe (Pyrenophora graminea), a seed-borne pathogen causing leaf stripe, was mapped with molecular markers in a barley doubled haploid (DH) population, derived from the cross Proctor x Nudinka (PN). The quantitative locus accounts for r2= 58.5% and was mapped on barley chromosome 1, tightly linked to the "naked" gene. A second resistance QTL, accounting for 29.3% of phenotypic variation, was identified on the P arm of barley chromosome 2. Another two minor QTLs were detected in further analyses. None of the QTLs were found in the chromosome 2 "vada" region studied by Giese et al. (1993).