The problem of estimating a classification rule with partially classified observations, which often occurs in biological and ecological modelling, and which is of major interest in pattern recognition, is discussed. Radial basis function networks for classification problems are presented and compared with the discriminant analysis with partially classified data, in situations where some observations in the training set are unclassified. An application on a set of morphometric data obtained from the skulls of 288 specimens of Microtus subterraneus and Microtus multiplex is performed. This example illustrates how the use of both classified and unclassified observations in the estimate of the hidden layer parameters has the potential to greatly improve the network performances.