A complete hobo-like element, called Mbhobo, was identified in the cabbage moth, Mamestra brassicae. This element has a high sequence similarity to the HFL1 hobo element of Drosophila melanogaster. Amplification of Mbhobo termini indicated that transposition occurred into a 5’-GTGGGTAC-3’ target sequence that was duplicated upon insertion. This target site conforms to the consensus sequence established for the insertion sites of insect hAT elements. Mbhobo has a single 1935 bp long ORF with significant homology to the D. melanogaster HFL1 hobo transposase. FISH experiments evidenced Mbhobo clusters located in heterochromatic regions of Z and W sex chromosomes and in heterochromatic areas of chromosome pair 10.

Identification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera) / Borsatti, Federica; Azzoni, Paola; Mandrioli, Mauro. - In: HEREDITAS. - ISSN 0018-0661. - STAMPA. - 139:(2004), pp. 151-155. [10.1111/j.1601-5223.2003.01768.x]

Identification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera).

BORSATTI, Federica;AZZONI, Paola;MANDRIOLI, Mauro
2004-01-01

Abstract

A complete hobo-like element, called Mbhobo, was identified in the cabbage moth, Mamestra brassicae. This element has a high sequence similarity to the HFL1 hobo element of Drosophila melanogaster. Amplification of Mbhobo termini indicated that transposition occurred into a 5’-GTGGGTAC-3’ target sequence that was duplicated upon insertion. This target site conforms to the consensus sequence established for the insertion sites of insect hAT elements. Mbhobo has a single 1935 bp long ORF with significant homology to the D. melanogaster HFL1 hobo transposase. FISH experiments evidenced Mbhobo clusters located in heterochromatic regions of Z and W sex chromosomes and in heterochromatic areas of chromosome pair 10.
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Identification of a new hobo element in the cabbage moth, Mamestra brassicae (Lepidoptera) / Borsatti, Federica; Azzoni, Paola; Mandrioli, Mauro. - In: HEREDITAS. - ISSN 0018-0661. - STAMPA. - 139:(2004), pp. 151-155. [10.1111/j.1601-5223.2003.01768.x]
Borsatti, Federica; Azzoni, Paola; Mandrioli, Mauro
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