WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38, a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed duringexposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38 genomic DNA was sequenced and mapped onto the centromeric region ofthe barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.

Hv-WRKY38: A New Transcription Factor Involved in Cold- and Drought-Response in Barley / C., Mare'; E., Mazzucotelli; C., Crosatti; Francia, Enrico; Stanca, ANTONIO MICHELE; L., Cattivelli. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - STAMPA. - 55:(2004), pp. 399-416.

Hv-WRKY38: A New Transcription Factor Involved in Cold- and Drought-Response in Barley

FRANCIA, Enrico;STANCA, ANTONIO MICHELE;
2004

Abstract

WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38, a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed duringexposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38 genomic DNA was sequenced and mapped onto the centromeric region ofthe barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.
55
399
416
Hv-WRKY38: A New Transcription Factor Involved in Cold- and Drought-Response in Barley / C., Mare'; E., Mazzucotelli; C., Crosatti; Francia, Enrico; Stanca, ANTONIO MICHELE; L., Cattivelli. - In: PLANT MOLECULAR BIOLOGY. - ISSN 0167-4412. - STAMPA. - 55:(2004), pp. 399-416.
C., Mare'; E., Mazzucotelli; C., Crosatti; Francia, Enrico; Stanca, ANTONIO MICHELE; L., Cattivelli
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/452187
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