WRKY proteins constitute a large family of plant specific transcription factors implicated in many different processes. Here we describe Hv-WRKY38, a barley gene coding for a WRKY protein, whose expression is involved in cold and drought stress response. Hv-WRKY38 was early and transiently expressed duringexposure to low non-freezing temperature, in ABA-independent manner. Furthermore, it showed a continuous induction during dehydration and freezing treatments. A WRKY38:YFP fusion protein was found to localise into the nucleus upon introduction into epidermal onion cells. Bacterially expressed Hv-WRKY38 was able to bind in vitro to the W-box element (T)TGAC(C/T) also recognisable by other WRKY proteins. Hv-WRKY38 genomic DNA was sequenced and mapped onto the centromeric region ofthe barley chromosome 6H. Arabidopsis and rice sequences homologous to Hv-WRKY38 were also identified. Our results indicate that Hv-WRKY38 transcription factor may play a regulatory role in abiotic stress response.
|Anno di pubblicazione:||2004|
|Titolo:||Hv-WRKY38: A New Transcription Factor Involved in Cold- and Drought-Response in Barley|
|Autori:||C. MARE'; E. MAZZUCOTELLI; C. CROSATTI; E. FRANCIA; AM. STANCA; L. CATTIVELLI|
|Appare nelle tipologie:||Articolo su rivista|
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