Bone and bone marrow are closely aligned physiologic compartments, suggesting that these tissues may represent a single functional unit with a common bone marrow progenitor that gives rise to both osteoblasts and hematopoietic cells. Although reports of multilineage engraftment by a single marrow-derived stem cell support this idea, more recent evidence has challenged claims of stem cell transdifferentiation and therefore the existence of a multipotent hematopoietic/osteogenic progenitor cell. Using a repopulation assay in mice, we show here that gene-marked, transplantable marrow cells from the plastic-nonadherent population can generate both functional osteoblasts/osteocytes and hematopoietic cells. Fluorescent in situ hybridization for the X and Y chromosomes and karyotype analysis of cultured osteoblasts confirmed the donor origin of these cells and excluded their generation by a fusion process. Molecular analysis demonstrated a common retroviral integration site in clonogenic hematopoietic cells and osteoprogenitors from each of seven animals studied, establishing a shared clonal origin for these ostensibly independent cell types. Our findings indicate that the bone marrow contains a primitive cell able to generate both the hematopoietic and osteocytic lineages. Its isolation and characterization may suggest novel treatments for genetic bone diseases and bone injuries.
|Data di pubblicazione:||2004|
|Titolo:||Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation|
|Autore/i:||Dominici, Massimo; C., Pritchard; J. E., Garlits; T. J., Hofmann; D. A., Persons; E. M., Horwitz|
|Codice identificativo ISI:||WOS:000223276700043|
|Codice identificativo Scopus:||2-s2.0-4143144309|
|Codice identificativo Pubmed:||15282377|
|Citazione:||Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation / Dominici, Massimo; C., Pritchard; J. E., Garlits; T. J., Hofmann; D. A., Persons; E. M., Horwitz. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - STAMPA. - 101(2004), pp. 11761-11766.|
|Tipologia||Articolo su rivista|
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