Bone and bone marrow are closely aligned physiologic compartments, suggesting that these tissues may represent a single functional unit with a common bone marrow progenitor that gives rise to both osteoblasts and hematopoietic cells. Although reports of multilineage engraftment by a single marrow-derived stem cell support this idea, more recent evidence has challenged claims of stem cell transdifferentiation and therefore the existence of a multipotent hematopoietic/osteogenic progenitor cell. Using a repopulation assay in mice, we show here that gene-marked, transplantable marrow cells from the plastic-nonadherent population can generate both functional osteoblasts/osteocytes and hematopoietic cells. Fluorescent in situ hybridization for the X and Y chromosomes and karyotype analysis of cultured osteoblasts confirmed the donor origin of these cells and excluded their generation by a fusion process. Molecular analysis demonstrated a common retroviral integration site in clonogenic hematopoietic cells and osteoprogenitors from each of seven animals studied, establishing a shared clonal origin for these ostensibly independent cell types. Our findings indicate that the bone marrow contains a primitive cell able to generate both the hematopoietic and osteocytic lineages. Its isolation and characterization may suggest novel treatments for genetic bone diseases and bone injuries.
|Anno di pubblicazione:||2004|
|Titolo:||Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation|
|Autore/i:||M. DOMINICI; C. PRITCHARD; J.E. GARLITS; T.J. HOFMANN; D.A. PERSONS; E.M. HORWITZ|
|Codice identificativo ISI:||WOS:000223276700043|
|Codice identificativo Scopus:||2-s2.0-4143144309|
|Tipologia||Articolo su rivista|
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