To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis

Development and functional characterization of human bone marrow mesenchymal cell immortalized by enforced expression of telomerase / K., Mihara; C., Imai; Coustan, Smith; J. S., DOME JS; Dominici, Massimo; E., Vanin; D., Campana. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - STAMPA. - 120:5(2003), pp. 846-849. [10.1046/j.1365-2141.2003.04217.x]

Development and functional characterization of human bone marrow mesenchymal cell immortalized by enforced expression of telomerase

DOMINICI, Massimo;
2003

Abstract

To create immortal mesenchymal cell lines, we transduced primary human bone marrow mesenchymal cells with telomerase reverse transcriptase (TERT). TERT+ mesenchymal cells continued to grow for > 2 years; parallel TERT- cultures underwent senescence after 15 weeks. TERT+ mesenchymal cells did not form foci in soft agar, had a normal karyotype and could differentiate into osteoblasts and chondrocytes. Their capacity to support leukaemic lymphoblasts and normal CD34+ haematopoietic cells was equal to or greater than that of primary cells; 42 TERT+ mesenchymal cell clones varied in their supporting capacity. Immortalized mesenchymal cells offer a promising tool for identifying molecules that regulate human haematopoiesis
2003
120
5
846
849
Development and functional characterization of human bone marrow mesenchymal cell immortalized by enforced expression of telomerase / K., Mihara; C., Imai; Coustan, Smith; J. S., DOME JS; Dominici, Massimo; E., Vanin; D., Campana. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - STAMPA. - 120:5(2003), pp. 846-849. [10.1046/j.1365-2141.2003.04217.x]
K., Mihara; C., Imai; Coustan, Smith; J. S., DOME JS; Dominici, Massimo; E., Vanin; D., Campana
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/451318
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