In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50 % of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.

Method for inducing experimental pneumococcal meningitis in outbred mice / Chiavolini, D; Tripodi, S; Parigi, R; Oggioni, M; Blasi, Elisabetta; Cintorino, M; Pozzi, G; Ricci, S.. - In: BMC MICROBIOLOGY. - ISSN 1471-2180. - STAMPA. - 4(2004), pp. 36-48.

Method for inducing experimental pneumococcal meningitis in outbred mice

BLASI, Elisabetta;
2004

Abstract

In the present work, we describe in detail a simple, reproducible and efficient method to induce pneumococcal meningitis in outbred mice by using the intracranial subarachnoidal route of infection. Bacteria were injected into the subarachnoid space through a soft point located 3.5 mm rostral from the bregma. The model was tested with several doses of pneumococci of three capsular serotypes (2, 3 and 4), and mice survival was recorded. Lethal doses killing 50 % of animals infected with type 2, 3 and 4 S. pneumoniae were 3.2 × 10, 2.9 × 10 and 1.9 × 102 colony forming units, respectively. Characterisation of the disease caused by the type 4 strain showed that in moribund mice systemic dissemination of pneumococci to blood and spleen occurred. Histological analysis of the brain of animals infected with type 4 S. pneumoniae proved the induction of meningitis closely resembling the disease in humans. The proposed method for inducing pneumococcal meningitis in outbred mice is easy-to-perform, fast, cost-effective, and reproducible, irrespective of the serotype of pneumococci used.
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Method for inducing experimental pneumococcal meningitis in outbred mice / Chiavolini, D; Tripodi, S; Parigi, R; Oggioni, M; Blasi, Elisabetta; Cintorino, M; Pozzi, G; Ricci, S.. - In: BMC MICROBIOLOGY. - ISSN 1471-2180. - STAMPA. - 4(2004), pp. 36-48.
Chiavolini, D; Tripodi, S; Parigi, R; Oggioni, M; Blasi, Elisabetta; Cintorino, M; Pozzi, G; Ricci, S.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/448279
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