Iron homeostasis and macrophage physiology are tightly intertwined. In the present study, we evaluated the influence of iron loading on the constitutive and interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS)-induced functional and secretory properties of microglial cells, using the in vitro established murine cell line BV-2. We demonstrate that iron augments the basal and IFN-gamma plus LPS-enhanced anti-Candida albicans activity exerted by BV-2 cells and that the phenomenon occurs with no enhancement of phagocytic activity. Furthermore, when the secretory properties of IFN-gamma plus LPS-treated BV-2 cells were assessed, we found that tumor necrosis factor remains unchanged while nitric oxide production is significantly reduced in iron-loaded cells. The addition of the iron chelator deferiprone (L1) reverts the effects of iron on BV-2 functional and secretory properties. These data suggest that iron differently affects secretory and effector functions of BV-2 microglial cells, thus implying that iron interferes with murine microglial cell physiology

Iron regulates microglial cell-mediated secretory and effector functions / Saleppico, S.; Mazzolla, R.; Boelaert, J. R.; Puliti, M.; Barluzzi, R.; Bistoni, F.; Blasi, Elisabetta. - In: CELLULAR IMMUNOLOGY. - ISSN 0008-8749. - ELETTRONICO. - 170:(1996), pp. 251-259.

Iron regulates microglial cell-mediated secretory and effector functions

BLASI, Elisabetta
1996

Abstract

Iron homeostasis and macrophage physiology are tightly intertwined. In the present study, we evaluated the influence of iron loading on the constitutive and interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS)-induced functional and secretory properties of microglial cells, using the in vitro established murine cell line BV-2. We demonstrate that iron augments the basal and IFN-gamma plus LPS-enhanced anti-Candida albicans activity exerted by BV-2 cells and that the phenomenon occurs with no enhancement of phagocytic activity. Furthermore, when the secretory properties of IFN-gamma plus LPS-treated BV-2 cells were assessed, we found that tumor necrosis factor remains unchanged while nitric oxide production is significantly reduced in iron-loaded cells. The addition of the iron chelator deferiprone (L1) reverts the effects of iron on BV-2 functional and secretory properties. These data suggest that iron differently affects secretory and effector functions of BV-2 microglial cells, thus implying that iron interferes with murine microglial cell physiology
170
251
259
Iron regulates microglial cell-mediated secretory and effector functions / Saleppico, S.; Mazzolla, R.; Boelaert, J. R.; Puliti, M.; Barluzzi, R.; Bistoni, F.; Blasi, Elisabetta. - In: CELLULAR IMMUNOLOGY. - ISSN 0008-8749. - ELETTRONICO. - 170:(1996), pp. 251-259.
Saleppico, S.; Mazzolla, R.; Boelaert, J. R.; Puliti, M.; Barluzzi, R.; Bistoni, F.; Blasi, Elisabetta
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/448262
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