The Saccharopolyspora erythraea genome sequence, recently published, presents considerable divergence from those of streptomycetes in gene organization and function, confirming the remarkable potential of S. erythraea for producing many other secondary metabolites in addition to erythromycin. In order to investigate, at whole transcriptome level, how S. erythraea genes are modulated, a DNA microarray was specifically designed and constructed on the S. erythraea strain NRRL 2338 genome sequence, and the expression profiles of 6494 ORFs were monitored during growth in complex liquid medium.
Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays / Peano, C; Bicciato, Silvio; Corti, G; Ferrari, F; Rizzi, E; Bonnal, Rj; Bordoni, R; Albertini, A; Bernardi, Lr; Donadio, S; DE BELLIS, G.. - In: MICROBIAL CELL FACTORIES. - ISSN 1475-2859. - ELETTRONICO. - 6:(2007), pp. 37-52. [10.1186/1475-2859-6-37]
Complete gene expression profiling of Saccharopolyspora erythraea using GeneChip DNA microarrays
BICCIATO, Silvio;
2007
Abstract
The Saccharopolyspora erythraea genome sequence, recently published, presents considerable divergence from those of streptomycetes in gene organization and function, confirming the remarkable potential of S. erythraea for producing many other secondary metabolites in addition to erythromycin. In order to investigate, at whole transcriptome level, how S. erythraea genes are modulated, a DNA microarray was specifically designed and constructed on the S. erythraea strain NRRL 2338 genome sequence, and the expression profiles of 6494 ORFs were monitored during growth in complex liquid medium.
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