TTo investigate the patterns of genetic lesions in a panel of 23 human multiple myeloma cell lines (HMCLs), we made a genomic integrative analysis involving FISH, and both gene expression and genome-wide profiling approaches. The expression profiles of the genes targeted by the main IGH translocations showed that the WHSCI/MMSET gene involved in t(4; 14)(p 16;q32) was expressed at different levels in all of the HMCL; and that the expression of the MAF gene was not restricted to the HMCLs carrying t(14;16)(q32;q23). Supervised analyses identified a limited number of genes specifically associated with t(4;14) and involved in different biological processes. The signature related to MAF/MAFB expression included the known MAF target genes CCND2 and ITGB7, as well as genes controlling cell shape and cell adhesion. Genome-wide DNA profiling allowed the identification of a gain on chromosome arm I q in 88% of the analyzed cell lines, together with recurrent gains on 8q, 18q, 7q, and 20q; the most frequent deletions affected I p, 13q, 17p, and 14q; and almost all of the cell lines presented LOH on chromosome 13. Two hundred and twenty-two genes were found to be simultaneously overexpressed and amplified in our panel, including the BCL2 locus at 18q21.33. Our data further support the evidence of the genomic complexity of multiple myeloma and reinforce the role of an integrated genomic approach in improving our understanding of the molecular pathogenesis of the disease.

Molecular characterization of human multiple myeloma cell lines by integrative genomics: Insights into the biology of the disease / Lombardi, L; Poretti, G; Mattioli, M; Fabris, S; Agnelli, L; Bicciato, Silvio; Kwee, I; Rinaldi, A; Ronchetti, D; Verdelli, D; LAMBERTENGHI DELILIERS, G; Bertoni, F; Neri, A.. - In: GENES, CHROMOSOMES & CANCER. - ISSN 1045-2257. - STAMPA. - 46:(2007), pp. 226-238. [10.1002/gcc.20404]

Molecular characterization of human multiple myeloma cell lines by integrative genomics: Insights into the biology of the disease

BICCIATO, Silvio;
2007

Abstract

TTo investigate the patterns of genetic lesions in a panel of 23 human multiple myeloma cell lines (HMCLs), we made a genomic integrative analysis involving FISH, and both gene expression and genome-wide profiling approaches. The expression profiles of the genes targeted by the main IGH translocations showed that the WHSCI/MMSET gene involved in t(4; 14)(p 16;q32) was expressed at different levels in all of the HMCL; and that the expression of the MAF gene was not restricted to the HMCLs carrying t(14;16)(q32;q23). Supervised analyses identified a limited number of genes specifically associated with t(4;14) and involved in different biological processes. The signature related to MAF/MAFB expression included the known MAF target genes CCND2 and ITGB7, as well as genes controlling cell shape and cell adhesion. Genome-wide DNA profiling allowed the identification of a gain on chromosome arm I q in 88% of the analyzed cell lines, together with recurrent gains on 8q, 18q, 7q, and 20q; the most frequent deletions affected I p, 13q, 17p, and 14q; and almost all of the cell lines presented LOH on chromosome 13. Two hundred and twenty-two genes were found to be simultaneously overexpressed and amplified in our panel, including the BCL2 locus at 18q21.33. Our data further support the evidence of the genomic complexity of multiple myeloma and reinforce the role of an integrated genomic approach in improving our understanding of the molecular pathogenesis of the disease.
46
226
238
Molecular characterization of human multiple myeloma cell lines by integrative genomics: Insights into the biology of the disease / Lombardi, L; Poretti, G; Mattioli, M; Fabris, S; Agnelli, L; Bicciato, Silvio; Kwee, I; Rinaldi, A; Ronchetti, D; Verdelli, D; LAMBERTENGHI DELILIERS, G; Bertoni, F; Neri, A.. - In: GENES, CHROMOSOMES & CANCER. - ISSN 1045-2257. - STAMPA. - 46:(2007), pp. 226-238. [10.1002/gcc.20404]
Lombardi, L; Poretti, G; Mattioli, M; Fabris, S; Agnelli, L; Bicciato, Silvio; Kwee, I; Rinaldi, A; Ronchetti, D; Verdelli, D; LAMBERTENGHI DELILIERS, G; Bertoni, F; Neri, A.
File in questo prodotto:
File Dimensione Formato  
Lombardi_2007_GCC.pdf

non disponibili

Tipologia: Versione dell'editore (versione pubblicata)
Dimensione 1.29 MB
Formato Adobe PDF
1.29 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/421438
Citazioni
  • ???jsp.display-item.citation.pmc??? 28
  • Scopus 59
  • ???jsp.display-item.citation.isi??? 57
social impact