Purpose: The developmental processes that mediate differentiation from retinal stem cells (RSC) to different retinal neuronal types remain unclear. During retinal development, progenitor cells modify expression of growth factor (GF) receptors and their differentiation potentials. Similarly, RSC in culture may exhibit alternative molecular characteristics in response to different GF stimuli.Methods: RSC were purified from the adult ciliary margin and exposed to fibroblast growth factor (FGF), epidermal growth factor (EGF), or FGF+EGF. Proliferation was analyzed by bromodeoxyuridine (BrdU) labeling. Differentiation was evaluated by immunofluorescence with antibodies recognizing specific markers of different retinal cell types.Results: In the absence of GF stimuli, RSC in culture expressed FGFR1, similar to early progenitors in vivo. Treatment with GFs up-regulated the expression of both fibroblast growth factor receptor 1 (FGFR1) and epidermal growth factor receptor (EGFR). Exposure to either FGF, EGF, or FGF+EGF strongly affected retinal stem cell-renewal and differentiation. Specifically, expression of progenitor/stem cell markers and stem cell-renewal was higher in the presence of FGF than in that of EGF. FGF favored differentiation of RSC into photoreceptor-like cells. Finally, we showed that the treatment of the primary culture with FGF+EGF imprinted the cells and limited plasticity in subsequent differentiation.Conclusions: We provide evidence that conditions of the primary culture have a strong influence on cell-renewal and differentiation potentials of RSC.

FGF and EGF differently affect differentiation of murine retinal stem cells in vitro / Giordano, F; DE MARZO, Anna; Vetrini, F; Marigo, Valeria. - In: MOLECULAR VISION. - ISSN 1090-0535. - STAMPA. - 13:(2007), pp. 1842-1850.

FGF and EGF differently affect differentiation of murine retinal stem cells in vitro

DE MARZO, Anna;MARIGO, Valeria
2007-01-01

Abstract

Purpose: The developmental processes that mediate differentiation from retinal stem cells (RSC) to different retinal neuronal types remain unclear. During retinal development, progenitor cells modify expression of growth factor (GF) receptors and their differentiation potentials. Similarly, RSC in culture may exhibit alternative molecular characteristics in response to different GF stimuli.Methods: RSC were purified from the adult ciliary margin and exposed to fibroblast growth factor (FGF), epidermal growth factor (EGF), or FGF+EGF. Proliferation was analyzed by bromodeoxyuridine (BrdU) labeling. Differentiation was evaluated by immunofluorescence with antibodies recognizing specific markers of different retinal cell types.Results: In the absence of GF stimuli, RSC in culture expressed FGFR1, similar to early progenitors in vivo. Treatment with GFs up-regulated the expression of both fibroblast growth factor receptor 1 (FGFR1) and epidermal growth factor receptor (EGFR). Exposure to either FGF, EGF, or FGF+EGF strongly affected retinal stem cell-renewal and differentiation. Specifically, expression of progenitor/stem cell markers and stem cell-renewal was higher in the presence of FGF than in that of EGF. FGF favored differentiation of RSC into photoreceptor-like cells. Finally, we showed that the treatment of the primary culture with FGF+EGF imprinted the cells and limited plasticity in subsequent differentiation.Conclusions: We provide evidence that conditions of the primary culture have a strong influence on cell-renewal and differentiation potentials of RSC.
13
1842
1850
FGF and EGF differently affect differentiation of murine retinal stem cells in vitro / Giordano, F; DE MARZO, Anna; Vetrini, F; Marigo, Valeria. - In: MOLECULAR VISION. - ISSN 1090-0535. - STAMPA. - 13:(2007), pp. 1842-1850.
Giordano, F; DE MARZO, Anna; Vetrini, F; Marigo, Valeria
File in questo prodotto:
File Dimensione Formato  
Giordano-MV.pdf

Open Access dal 02/01/2008

Tipologia: Versione pubblicata dall'editore
Dimensione 564.02 kB
Formato Adobe PDF
564.02 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/421327
Citazioni
  • ???jsp.display-item.citation.pmc??? 10
  • Scopus 35
  • ???jsp.display-item.citation.isi??? 29
social impact