The present case report was aimed at identifying the molecular profile characteristic of a primitive neuroectodermal tumor (PNET) in a 3-year-old child affected by a lesion localized in the cerebellar region. The histological diagnosis was medulloblastoma. In vivo single voxel H-1 magnetic resonance spectroscopy (MRS) shows high specificity in detecting the main metabolic alterations in the primitive cerebellar lesion; a very high amount of the choline-containing compounds and very low level of creatine derivatives and N-acetylaspartate. Ex vivo high resolution magic angle spinning (HR-MAS) H-1 magnetic resonance spectroscopy, performed at 9.4 Tesla on the neoplastic specimen collected during surgery, allows for the unambiguous identification of several metabolites giving a more in-depth evaluation of the metabolic pattern of the lesion. The ex vivo HR-MAS MR spectra show that the spectral detail is much higher than that obtained in vivo and that, for example, myo-inositol, taurine and phosphorylethanolamine contribute to the in vivo signal at 3.2 ppm, usually attributed to choline-containing compounds. In addition, the spectroscopic data appear to correlate with some morphological features of the medulloblastoma. Consequently, the present study shows that ex vivo HR-MAS H-1 MRS is able to strongly improve the clinical possibility of in vivo MRS and can be used in conjunction with in vivo spectroscopy for clinical purposes.

A comparison between in vivo and ex vivo HR-MAS H-1 MR spectra of a pediatric posterior fossa lesion / Tugnoli, V; Schenetti, Luisa; Mucci, Adele; Nocetti, L; Toraci, C; Mavilla, L; Basso, G; Rovati, R; Tavani, F; Zunarelli, E; Righi, V; Tosi, Mr. - In: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. - ISSN 1107-3756. - STAMPA. - 16:(2005), pp. 301-307.

A comparison between in vivo and ex vivo HR-MAS H-1 MR spectra of a pediatric posterior fossa lesion

SCHENETTI, Luisa;MUCCI, Adele;
2005-01-01

Abstract

The present case report was aimed at identifying the molecular profile characteristic of a primitive neuroectodermal tumor (PNET) in a 3-year-old child affected by a lesion localized in the cerebellar region. The histological diagnosis was medulloblastoma. In vivo single voxel H-1 magnetic resonance spectroscopy (MRS) shows high specificity in detecting the main metabolic alterations in the primitive cerebellar lesion; a very high amount of the choline-containing compounds and very low level of creatine derivatives and N-acetylaspartate. Ex vivo high resolution magic angle spinning (HR-MAS) H-1 magnetic resonance spectroscopy, performed at 9.4 Tesla on the neoplastic specimen collected during surgery, allows for the unambiguous identification of several metabolites giving a more in-depth evaluation of the metabolic pattern of the lesion. The ex vivo HR-MAS MR spectra show that the spectral detail is much higher than that obtained in vivo and that, for example, myo-inositol, taurine and phosphorylethanolamine contribute to the in vivo signal at 3.2 ppm, usually attributed to choline-containing compounds. In addition, the spectroscopic data appear to correlate with some morphological features of the medulloblastoma. Consequently, the present study shows that ex vivo HR-MAS H-1 MRS is able to strongly improve the clinical possibility of in vivo MRS and can be used in conjunction with in vivo spectroscopy for clinical purposes.
16
301
307
A comparison between in vivo and ex vivo HR-MAS H-1 MR spectra of a pediatric posterior fossa lesion / Tugnoli, V; Schenetti, Luisa; Mucci, Adele; Nocetti, L; Toraci, C; Mavilla, L; Basso, G; Rovati, R; Tavani, F; Zunarelli, E; Righi, V; Tosi, Mr. - In: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. - ISSN 1107-3756. - STAMPA. - 16:(2005), pp. 301-307.
Tugnoli, V; Schenetti, Luisa; Mucci, Adele; Nocetti, L; Toraci, C; Mavilla, L; Basso, G; Rovati, R; Tavani, F; Zunarelli, E; Righi, V; Tosi, Mr
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/3485
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