Recently, we have shown a differential susceptibility of non-pathogenic vs. pathogenic leptospires to phagocytosis and killing by microglial cells. Although all ingested to some extent, only the pathogenic strains survived intracellularly while the non-pathogenic ones were killed in a time-dependent manner. By the same infection model, here we demonstrate that microglial cells respond to Leptospira infection with a time- and dose-dependent induction of molecular signals (p38 phosphorilation and NF-kB activation) and the production of soluble factors (cytokines and nitric oxide). Such bio-molecular response is predominantly observed against the pathogenic Leptospira; the phenomenon is reproduced by leptospiral lipoproteins and, to a lower extent, by leptospiral-derived LPS. These data provide initial evidence that Leptospira affects microglial cell response in a different manner depending upon the virulence of the infecting strain; specific bacterial components happen to be involved in the induction of such pathogen-induced immune response.

NF-kB activation and p38 phosphorilation in microglial cells infected with Leptospira or exposed to partially purified leptospiral lipoproteins / Blasi, Elisabetta; Ardizzoni, Andrea; Colombari, Bruna; Neglia, Rachele Giovanna; Baschieri, MARIA CRISTINA; Peppoloni, Samuele; M., Cinco. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - STAMPA. - 42 (2-3):(2007), pp. 80-87.

NF-kB activation and p38 phosphorilation in microglial cells infected with Leptospira or exposed to partially purified leptospiral lipoproteins

BLASI, Elisabetta;ARDIZZONI, Andrea;COLOMBARI, Bruna;NEGLIA, Rachele Giovanna;BASCHIERI, MARIA CRISTINA;PEPPOLONI, Samuele;
2007

Abstract

Recently, we have shown a differential susceptibility of non-pathogenic vs. pathogenic leptospires to phagocytosis and killing by microglial cells. Although all ingested to some extent, only the pathogenic strains survived intracellularly while the non-pathogenic ones were killed in a time-dependent manner. By the same infection model, here we demonstrate that microglial cells respond to Leptospira infection with a time- and dose-dependent induction of molecular signals (p38 phosphorilation and NF-kB activation) and the production of soluble factors (cytokines and nitric oxide). Such bio-molecular response is predominantly observed against the pathogenic Leptospira; the phenomenon is reproduced by leptospiral lipoproteins and, to a lower extent, by leptospiral-derived LPS. These data provide initial evidence that Leptospira affects microglial cell response in a different manner depending upon the virulence of the infecting strain; specific bacterial components happen to be involved in the induction of such pathogen-induced immune response.
42 (2-3)
80
87
NF-kB activation and p38 phosphorilation in microglial cells infected with Leptospira or exposed to partially purified leptospiral lipoproteins / Blasi, Elisabetta; Ardizzoni, Andrea; Colombari, Bruna; Neglia, Rachele Giovanna; Baschieri, MARIA CRISTINA; Peppoloni, Samuele; M., Cinco. - In: MICROBIAL PATHOGENESIS. - ISSN 0882-4010. - STAMPA. - 42 (2-3):(2007), pp. 80-87.
Blasi, Elisabetta; Ardizzoni, Andrea; Colombari, Bruna; Neglia, Rachele Giovanna; Baschieri, MARIA CRISTINA; Peppoloni, Samuele; M., Cinco
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/310860
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