Cultures of isolated neuronal populations from the central ganglia of the gastropod mollusc Planorbarius corneus were used for testing the effects of inorganic lead. The examined parameters were cell survival, neurite outgrowth and cytoskeletal morphology. In large heterogeneous neuronal populations as obtained from a whole cerebral or pedal ganglion, the different sensitivity to lead is reflected mainly on the cell survival. The neurons belonging to the homogeneous E cluster population are more sensitive; in fact a higher percentage of them do not survive in the presence of lead. Moreover, in this neuronal cell type the neurite outgrowth is dramatically affected by lead only when the neurons are cultured on conditioned substrate. Possibly, membrane mechanisms activated for the neurite outgrowth represent a target for inorganic lead. The few neurites sprouted in presence of lead do not evidence changes in the cytoskeletal components.

Cell-specific effects of lead on cultured neurons of the freshwater snail Planorbarius corneus / F., Bianchi; Amf, Bolognani; Fratello, Bernardo; Sabatini, Maria Agnese; Sonetti, Dario. - In: ACTA BIOLOGICA HUNGARICA. - ISSN 0236-5383. - STAMPA. - 44:(1993), pp. 15-19.

Cell-specific effects of lead on cultured neurons of the freshwater snail Planorbarius corneus

FRATELLO, Bernardo;SABATINI, Maria Agnese;SONETTI, Dario
1993-01-01

Abstract

Cultures of isolated neuronal populations from the central ganglia of the gastropod mollusc Planorbarius corneus were used for testing the effects of inorganic lead. The examined parameters were cell survival, neurite outgrowth and cytoskeletal morphology. In large heterogeneous neuronal populations as obtained from a whole cerebral or pedal ganglion, the different sensitivity to lead is reflected mainly on the cell survival. The neurons belonging to the homogeneous E cluster population are more sensitive; in fact a higher percentage of them do not survive in the presence of lead. Moreover, in this neuronal cell type the neurite outgrowth is dramatically affected by lead only when the neurons are cultured on conditioned substrate. Possibly, membrane mechanisms activated for the neurite outgrowth represent a target for inorganic lead. The few neurites sprouted in presence of lead do not evidence changes in the cytoskeletal components.
44
15
19
Cell-specific effects of lead on cultured neurons of the freshwater snail Planorbarius corneus / F., Bianchi; Amf, Bolognani; Fratello, Bernardo; Sabatini, Maria Agnese; Sonetti, Dario. - In: ACTA BIOLOGICA HUNGARICA. - ISSN 0236-5383. - STAMPA. - 44:(1993), pp. 15-19.
F., Bianchi; Amf, Bolognani; Fratello, Bernardo; Sabatini, Maria Agnese; Sonetti, Dario
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/310587
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact