Objective: To evaluate the effect of a continuous combined oral hormone replacement therapy (HRT) on basal and post-methionine load homocysteine levels in postmenopausal women. Design: Twenty-two postmenopausal women (PMW) were randomly allocated to receive either continuous combined oral HRT (2 mg of estradiol plus 1 mg of norethisterone acetate; n = 11) or no treatment (controls, n = 11) for 6 months. A methionine oral load (0.1 g/kg body weight) was performed in each subject at time 0 and after 6 months. Serum homocysteine levels were measured by high-performance liquid chromatography in samples collected at time 0 and at 4, 8, and 24 h after the methionine load, while levels of vitamin B, (by high-performance liquid chromatography) and B,, and folate (both by ELISA) were assayed in samples collected at time 0. Results: Serum levels of glucose and body mass index increased in treated PMW, whereas folate decreased in controls. In treated PMW, basal homocysteine tended to decrease (10.6 +/- 3.3 mu mol/L vs. 9.62 +/- 2.8 mu mol/L, p = 0.062), whereas in controls it significantly increased (10.7 +/- 2.65 mu mol/L vs. 12.17 +/- 3.89 mu mol/L, p < 0.05). This increase was not significant after correction for vitamin status (p = 0.072). Homocysteine values 4 h (31.9 +/- 13.53 mu mol/L vs. 39.83 +/- 22.53 mu mol/L, p < 0.05) and 8 h (35.1 +/- 13.13 vs. 43.34 +/- 22.15 mu mol/L) after methionine, and integrated homocysteine response to methionine (392.5 +/- 133.8 mu mol/24 h vs. 458.8 +/- 104.8 mu mol/24 h; p < 0.05), were significantly reduced in HRT-treated, but not in untreated, PMW. Conclusions: Continuous combined oral HRT with 17 beta -estradiol plus norethisterone acetate reduces homocysteine levels, mainly after a methionine load. This effect seems to be independent of vitamin status and may have positive implications for the prevention of cardiovascular diseases in PMW.

Continuous combined hormone replacement therapy with oral 17 beta-estradiol and norethisterone acetate improves homocysteine metabolism in postmenopausal women / Ventura, Paolo; Cagnacci, Angelo; S., Malmusi; R., Panini; F., Baldassari; S., Arangino; Volpe, Annibale; Salvioli, Gianfranco. - In: MENOPAUSE. - ISSN 1072-3714. - STAMPA. - 8:(2001), pp. 252-258.

Continuous combined hormone replacement therapy with oral 17 beta-estradiol and norethisterone acetate improves homocysteine metabolism in postmenopausal women

VENTURA, Paolo;CAGNACCI, Angelo;VOLPE, Annibale;SALVIOLI, Gianfranco
2001

Abstract

Objective: To evaluate the effect of a continuous combined oral hormone replacement therapy (HRT) on basal and post-methionine load homocysteine levels in postmenopausal women. Design: Twenty-two postmenopausal women (PMW) were randomly allocated to receive either continuous combined oral HRT (2 mg of estradiol plus 1 mg of norethisterone acetate; n = 11) or no treatment (controls, n = 11) for 6 months. A methionine oral load (0.1 g/kg body weight) was performed in each subject at time 0 and after 6 months. Serum homocysteine levels were measured by high-performance liquid chromatography in samples collected at time 0 and at 4, 8, and 24 h after the methionine load, while levels of vitamin B, (by high-performance liquid chromatography) and B,, and folate (both by ELISA) were assayed in samples collected at time 0. Results: Serum levels of glucose and body mass index increased in treated PMW, whereas folate decreased in controls. In treated PMW, basal homocysteine tended to decrease (10.6 +/- 3.3 mu mol/L vs. 9.62 +/- 2.8 mu mol/L, p = 0.062), whereas in controls it significantly increased (10.7 +/- 2.65 mu mol/L vs. 12.17 +/- 3.89 mu mol/L, p < 0.05). This increase was not significant after correction for vitamin status (p = 0.072). Homocysteine values 4 h (31.9 +/- 13.53 mu mol/L vs. 39.83 +/- 22.53 mu mol/L, p < 0.05) and 8 h (35.1 +/- 13.13 vs. 43.34 +/- 22.15 mu mol/L) after methionine, and integrated homocysteine response to methionine (392.5 +/- 133.8 mu mol/24 h vs. 458.8 +/- 104.8 mu mol/24 h; p < 0.05), were significantly reduced in HRT-treated, but not in untreated, PMW. Conclusions: Continuous combined oral HRT with 17 beta -estradiol plus norethisterone acetate reduces homocysteine levels, mainly after a methionine load. This effect seems to be independent of vitamin status and may have positive implications for the prevention of cardiovascular diseases in PMW.
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Continuous combined hormone replacement therapy with oral 17 beta-estradiol and norethisterone acetate improves homocysteine metabolism in postmenopausal women / Ventura, Paolo; Cagnacci, Angelo; S., Malmusi; R., Panini; F., Baldassari; S., Arangino; Volpe, Annibale; Salvioli, Gianfranco. - In: MENOPAUSE. - ISSN 1072-3714. - STAMPA. - 8:(2001), pp. 252-258.
Ventura, Paolo; Cagnacci, Angelo; S., Malmusi; R., Panini; F., Baldassari; S., Arangino; Volpe, Annibale; Salvioli, Gianfranco
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/310582
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