In this study we have investigated the chemical-physical and morphological properties of intact and atelocollagen sponges used for tissue engineering. The porous sponges were prepared by lyophilization and their physico-chemical characteristics (water binding capacity, denaturing temperature, amino group content) were investigated. Considering the importance of the “in vivo” interactions between these sponges and the tissue, our attention was addressed a) to clarify the relationships between the morphology and the amount of water absorbed and b) to evaluate the influence of pepsin-alkaline treatment on the reorganization of the atelocollagen fibres. Conventional scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were employed to study the morphology and wetting behaviour of the intact and atelocollagen sponges. The observations by SEM indicated remarkable differences both in the structure and dimension of the pores between intact and atelocollagen sponges. At the data are related to a different water binding capacity. However, the ESEM observations, achieved by changing the relative humidity in the operative chamber, demonstrated that the water adsorbed can be removed with major difficulty from atelocollagen sponges than from intact ones

Intact collagen and atelocollagen sponges: Characterization and ESEM observation / Ruozi, Barbara; Tosi, Giovanni; Leo, Eliana Grazia; B., Parma; S., Vismara; Forni, Flavio; Vandelli, Maria Angela. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - STAMPA. - 27:(2007), pp. 802-810. [10.1016/j.msec.2006.08.018]

Intact collagen and atelocollagen sponges: Characterization and ESEM observation

RUOZI, Barbara;TOSI, Giovanni;LEO, Eliana Grazia;FORNI, Flavio;VANDELLI, Maria Angela
2007-01-01

Abstract

In this study we have investigated the chemical-physical and morphological properties of intact and atelocollagen sponges used for tissue engineering. The porous sponges were prepared by lyophilization and their physico-chemical characteristics (water binding capacity, denaturing temperature, amino group content) were investigated. Considering the importance of the “in vivo” interactions between these sponges and the tissue, our attention was addressed a) to clarify the relationships between the morphology and the amount of water absorbed and b) to evaluate the influence of pepsin-alkaline treatment on the reorganization of the atelocollagen fibres. Conventional scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were employed to study the morphology and wetting behaviour of the intact and atelocollagen sponges. The observations by SEM indicated remarkable differences both in the structure and dimension of the pores between intact and atelocollagen sponges. At the data are related to a different water binding capacity. However, the ESEM observations, achieved by changing the relative humidity in the operative chamber, demonstrated that the water adsorbed can be removed with major difficulty from atelocollagen sponges than from intact ones
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802
810
Intact collagen and atelocollagen sponges: Characterization and ESEM observation / Ruozi, Barbara; Tosi, Giovanni; Leo, Eliana Grazia; B., Parma; S., Vismara; Forni, Flavio; Vandelli, Maria Angela. - In: MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS. - ISSN 0928-4931. - STAMPA. - 27:(2007), pp. 802-810. [10.1016/j.msec.2006.08.018]
Ruozi, Barbara; Tosi, Giovanni; Leo, Eliana Grazia; B., Parma; S., Vismara; Forni, Flavio; Vandelli, Maria Angela
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/310499
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