EnglishA sensitive and reproducible 96-well assay of uronic acid permitting a rapid processing of a number of samples with a very low consumption of reagents is described-for the determination of complex uronic acid-bearing polyanions such as hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparin. The sensitivity of the reaction was approx. 1 mug for glucuronic acid and 2 mug for complex polysaccharides, with a linear function of glucuronic acid concentration between 1 and 100 mug. The relative coefficient of variations ranged from 1.5 to 8.7% for the assay performed in the 96-well plate. These values were found to be lower than those obtained by the conventional procedure.
A 96-well assay for uronic acid carbazole reaction / M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 54(2003), pp. 59-61.
| Data di pubblicazione: | 2003 |
| Titolo: | A 96-well assay for uronic acid carbazole reaction |
| Autore/i: | M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola |
| Autore/i UNIMORE: | |
| Rivista: | |
| Volume: | 54 |
| Pagina iniziale: | 59 |
| Pagina finale: | 61 |
| Codice identificativo ISI: | WOS:000185071800008 |
| Codice identificativo Scopus: | 2-s2.0-0042029363 |
| Citazione: | A 96-well assay for uronic acid carbazole reaction / M., Cesaretti; E., Luppi; Maccari, Francesca; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 54(2003), pp. 59-61. |
| Tipologia | Articolo su rivista |
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