Two cell populations with different phenol-phenotypes were selected from leaf explants of the ozone-sensitive Vitis vinifera L. cv. Lambrusco salamino. Experimental exposure of these cell lines to a 2 h fumigation with 300 ppb ozone caused a differential response. Peroxidative injuries, evaluated on the basis of malondialdehyde production and at the ultrastructural level, were induced in phenol-rich (Ph+) but not in phenol-poorer (Ph-) cells. Apoplastic ascorbate-peroxidase (EC 18.104.22.168), to which a prevailing role in apoplastic H2O2 scavenging has been attributed, was substantially stimulated in Ph- cells during ozone exposure; guaiacol-peroxidase (EC 22.214.171.124) reached maximum values 12 h later. Only guaiacol-peroxidase was rapidly induced in Ph+ cells, whereas ascorbate-peroxidase was inhibited to some extent. Phenol-poor cells thus proved able to promptly activate the biphasic extracellular peroxidase(s) defence system against ozone induced oxidative stress. On the contrary, the phenol-rich cell type appeared to be a very sensitive target of ozone toxicity; this suggests that the sensitivity displayed by the whole leaf of the grape cultivar used as explant source might be conditioned by the constitutive presence of a great number of such cells.
|Anno di pubblicazione:||1999|
|Titolo:||Differential sensitivity to ozone in two selected cell lines from grape leaf|
|Autori:||E. SGARBI; P. BONATTI; R. BARONI FORNASIERO; A. LINS|
|Appare nelle tipologie:||Articolo su rivista|
File in questo prodotto:
I documenti presenti in Iris Unimore sono rilasciati con licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 3.0 Italia, salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris