Using congenic strains of mice susceptible (bcg(s)) or resistant (bcg(r)) to BCG, murine microglial cell lines, RR4.R (BCG-resistant) and RR8.S (BCG-susceptible), were established in vitro. Comparative studies revealed that, although phagocytic to a similar extent, RR4.R cells were more active than RR8.S cells in terms of antimycobacterial activity. Interestingly, cells of resistant genotype secreted more nitric oxide, TNF-alpha and IL-12, but less IL-6, than susceptible cells, when stimulated with IFN-gamma alone or in combination with lipolysaccharide. Nevertheless, no significant differences were observed between the two cell lines in terms of IL-1 beta or IL-10 secretion, or on assessment of cytokine production following exposure to a massive dose of Lipopolysaccharide. Overall, these data provide the first evidence that resistant/susceptible genotype influences antimycobacterial activity, NO and cytokine production in microglial cells, the prototype of cerebral macrophages. (C) 1999 Elsevier Science B.V. All rights reserved.

Differential effector and secretory functions of microglial cell lines derived from BCG-resistant and -susceptible congenic mouse strains / M., Puliti; R., Mazzolla; A., Brozzetti; Neglia, Rachele Giovanna; D., Radzioch; F., Bistoni; Blasi, Elisabetta. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 101:(1999), pp. 27-33.

Differential effector and secretory functions of microglial cell lines derived from BCG-resistant and -susceptible congenic mouse strains

NEGLIA, Rachele Giovanna;BLASI, Elisabetta
1999

Abstract

Using congenic strains of mice susceptible (bcg(s)) or resistant (bcg(r)) to BCG, murine microglial cell lines, RR4.R (BCG-resistant) and RR8.S (BCG-susceptible), were established in vitro. Comparative studies revealed that, although phagocytic to a similar extent, RR4.R cells were more active than RR8.S cells in terms of antimycobacterial activity. Interestingly, cells of resistant genotype secreted more nitric oxide, TNF-alpha and IL-12, but less IL-6, than susceptible cells, when stimulated with IFN-gamma alone or in combination with lipolysaccharide. Nevertheless, no significant differences were observed between the two cell lines in terms of IL-1 beta or IL-10 secretion, or on assessment of cytokine production following exposure to a massive dose of Lipopolysaccharide. Overall, these data provide the first evidence that resistant/susceptible genotype influences antimycobacterial activity, NO and cytokine production in microglial cells, the prototype of cerebral macrophages. (C) 1999 Elsevier Science B.V. All rights reserved.
101
27
33
Differential effector and secretory functions of microglial cell lines derived from BCG-resistant and -susceptible congenic mouse strains / M., Puliti; R., Mazzolla; A., Brozzetti; Neglia, Rachele Giovanna; D., Radzioch; F., Bistoni; Blasi, Elisabetta. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - STAMPA. - 101:(1999), pp. 27-33.
M., Puliti; R., Mazzolla; A., Brozzetti; Neglia, Rachele Giovanna; D., Radzioch; F., Bistoni; Blasi, Elisabetta
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/308008
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