OBJECTIVE: To investigate the ex vivo platelet aggregation in response to a substrate (L-arginine [L-Arg]) and a donor (sodium nitroprusside [SN]) of nitric oxide (NO) in nonpregnant women and in normotensive pregnancies. METHODS: Platelet aggregation was studied with a dual-channel aggregometer and expressed as a percentage of light transmission. Measurements were done at baseline and after preincubation with scalar doses of L-Arg and SN. The intraplatelet L-citrulline (L-Cit) levels were measured by high-performance liquid chromotography (HPLC). RESULTS: In both groups, the baseline aggregation values were similar for adenosine diphosphate (ADP) and collagen stimuli. Wilcoxon tank-sum testing demonstrated that in both groups the addition of L-Arg had a significant effect on aggregation: Doses of 50 to 5000 mu mol decreased ADP-induced aggregation. Conversely, collagen-induced aggregation was affected only by the highest dose of L-Arg. Sodium nitroprusside administered in doses of 2.5 to 250.0 nmol decreased ADP- and collagen-induced platelet aggregation equally. ADP-induced aggregation values obtained after (SN) incubation were positively correlated with gestational age. Intraplatelet L-Cit levels showed a significant rise after the incubation with L-Arg, and this effect was negatively correlated with gestational age. CONCLUSION: The L-Arg-NO system regulates platelet aggregation during pregnancy. Moreover, a physiologic reduction of platelet sensitivity to the antithrombotic effect of NO occurs. (J Soc Gynecol Invest 1998; 5:192-196). Copyright (C) 1998 by the Society for Gynecologic Investigation.

The L-arginine-nitric oxide system regulates platelet aggregation in pregnancy / Neri, Isabella; M., Marietta; F., Piccinini; Volpe, Annibale; Facchinetti, Fabio. - In: JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION. - ISSN 1071-5576. - STAMPA. - 5:(1998), pp. 192-196.

The L-arginine-nitric oxide system regulates platelet aggregation in pregnancy

NERI, Isabella;VOLPE, Annibale;FACCHINETTI, Fabio
1998-01-01

Abstract

OBJECTIVE: To investigate the ex vivo platelet aggregation in response to a substrate (L-arginine [L-Arg]) and a donor (sodium nitroprusside [SN]) of nitric oxide (NO) in nonpregnant women and in normotensive pregnancies. METHODS: Platelet aggregation was studied with a dual-channel aggregometer and expressed as a percentage of light transmission. Measurements were done at baseline and after preincubation with scalar doses of L-Arg and SN. The intraplatelet L-citrulline (L-Cit) levels were measured by high-performance liquid chromotography (HPLC). RESULTS: In both groups, the baseline aggregation values were similar for adenosine diphosphate (ADP) and collagen stimuli. Wilcoxon tank-sum testing demonstrated that in both groups the addition of L-Arg had a significant effect on aggregation: Doses of 50 to 5000 mu mol decreased ADP-induced aggregation. Conversely, collagen-induced aggregation was affected only by the highest dose of L-Arg. Sodium nitroprusside administered in doses of 2.5 to 250.0 nmol decreased ADP- and collagen-induced platelet aggregation equally. ADP-induced aggregation values obtained after (SN) incubation were positively correlated with gestational age. Intraplatelet L-Cit levels showed a significant rise after the incubation with L-Arg, and this effect was negatively correlated with gestational age. CONCLUSION: The L-Arg-NO system regulates platelet aggregation during pregnancy. Moreover, a physiologic reduction of platelet sensitivity to the antithrombotic effect of NO occurs. (J Soc Gynecol Invest 1998; 5:192-196). Copyright (C) 1998 by the Society for Gynecologic Investigation.
5
192
196
The L-arginine-nitric oxide system regulates platelet aggregation in pregnancy / Neri, Isabella; M., Marietta; F., Piccinini; Volpe, Annibale; Facchinetti, Fabio. - In: JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION. - ISSN 1071-5576. - STAMPA. - 5:(1998), pp. 192-196.
Neri, Isabella; M., Marietta; F., Piccinini; Volpe, Annibale; Facchinetti, Fabio
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/307600
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 13
social impact