Oxidant/antioxidant imbalance is implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD). The current study examined the expression of antioxidant and pro-oxidant enzymes, haem oxygenases; (140) and inducible nitric oxide synthase (iNOS) respectively, in patients with severe COPD and control smokers without lung function impairment. Immunoreactivity for HO-1, HO-2, iNOS and nitric oxide-derived oxidants expressed as nitrotyrosine (N-Tyr) was quantified in peripheral lung. HO-1+ alveolar macrophages were decreased in severe COPD compared to control smokers, whereas no difference was observed in iNOS+ macrophages. In contrast, severe patients had significantly higher numbers of iNOS+ cells in alveolar walls. These iNOS+ cells were identified as type 2 pneumocytes and their number was inversely related to HO-1+ macrophages. There were no significant differences in N-Tyr immunostaining between the two groups. However, the rate of protein nitration in lung tissue was directly related to iNOS expression and associated with lower values of forced expiratory volume in one second/forced vital capacity. HO-2 was constitutively expressed by type 2 pneumocytes and these cells were increased in severe COPD. In conclusion, the results suggest that the enzymes involved in the oxidative stress response may have a different role in the lung defence and that imbalance between haem oxygenase-1 and inducible nitric oxide synthase may be associated with the development of severe impairment in chronic obstructive pulmonary disease patients.
|Data di pubblicazione:||2003|
|Titolo:||Decreased haem oxygenase-1 and increased inducible nitric oxide synthase in the lung of severe COPD patients|
|Autore/i:||P., Maestrelli; C., Paska; M., Saetta; G., Turato; Y., Nowicki; S., Monti; B., Formichi; M., Miniati; Fabbri, Leonardo|
|Codice identificativo ISI:||WOS:000183242900011|
|Codice identificativo Scopus:||2-s2.0-0037975679|
|Codice identificativo Pubmed:||12797490|
|Citazione:||Decreased haem oxygenase-1 and increased inducible nitric oxide synthase in the lung of severe COPD patients / P., Maestrelli; C., Paska; M., Saetta; G., Turato; Y., Nowicki; S., Monti; B., Formichi; M., Miniati; Fabbri, Leonardo. - In: EUROPEAN RESPIRATORY JOURNAL. - ISSN 0903-1936. - STAMPA. - 21(2003), pp. 971-976.|
|Tipologia||Articolo su rivista|
File in questo prodotto:
I documenti presenti in Iris Unimore sono rilasciati con licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 3.0 Italia, salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris