Keratinocytes, a key cellular component both for homeostasis and pathophysiologic processes of the skin, secrete a number of cytokines and are stimulated by several growth factors. Nerve growth factor (NGF) is synthesized in the skin and basal keratinocytes express the low-affinity nerve growth factor receptor (NGF-R). We present evidence that normal human keratinocytes in culture express the low- and the high-affinity NGF-R both at the mRNA level, as determined by reverse-transcription polymerase chain reaction and at the protein level, as shown by cytofluorimetric analysis. NGF significantly stimulates the proliferation of normal human keratinocytes in culture in a dose-dependent manner. This effect can be prevented by the addition of both an anti-NGF neutralizing antibody and a high-affinity NGF-R (trk) specific inhibitor, the natural alkaloid K252a. By contrast, keratinocyte proliferation is not inhibited by an anti - low-affinity NGF-R monoclonal antibody, thus suggesting that NGF effect on human keratinocytes is mediated by the high-affinity NGF-R. Moreover, NGF mRNA is expressed in normal human keratinocytes and NGF is secreted by keratinocytes in increasing amounts during growth, as detected by enzyme-linked immunosorbent assay. These results suggest that NGF could act as a cytokine in human skin and take part in disorders of keratinocyte proliferation.
|Anno di pubblicazione:||1994|
|Titolo:||Expression And Function Of Nerve Growth-Factor And Nerve Growth-Factor Receptor On Cultured Keratinocytes.|
|Autore/i:||C. Pincelli; C. Sevignani; R. Manfredini; A. Grande; F. Fantini; L. Bracci-Laudiero; L. Aloe; S. Ferrari; A. Cossarizza; A. Giannetti|
|Codice identificativo ISI:||WOS:A1994NW53300003|
|Codice identificativo Scopus:||2-s2.0-0028304787|
|Citazione:||Expression And Function Of Nerve Growth-Factor And Nerve Growth-Factor Receptor On Cultured Keratinocytes. / C. Pincelli; C. Sevignani; R. Manfredini; A. Grande; F. Fantini; L. Bracci-Laudiero; L. Aloe; S. Ferrari; A. Cossarizza; A. Giannetti. - In: JOURNAL OF INVESTIGATIVE DERMATOLOGY. - ISSN 0022-202X. - STAMPA. - 103(1994), pp. 13-18.|
|Tipologia||Articolo su rivista|
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