NF-Y is a trimeric activator with histone fold - HFM - subunits that binds to the CCAAT-box and is required for a majority of cell cycle promoters, often in conjuction with E2Fs. In vivo binding of NF-Y is dynamic during the cell cycle and correlates with gene activation. We performed immunofluorescence studies on endogenous, GFP- and Flag-tagged overexpressed NF-Y subunits. NF-YA, NF-YB are nuclear proteins. Unexpectedly, NF-YC localizes both in cytoplamatic and nuclear compartments and its nuclear localization is determined by the interaction with its heterodimerization partner NF-YB. Most importantly, compartmentalization is regulated during the cell cycle of serum restimulated NIH3T3 cells, accumulating in the nucleus at the onset of S phase. These data point to the control of HFM heterodimerization as an important layer of NF-Y regulation during cell cycle progression.

Cell cycle regulation of NF-YC nuclear localization / M., Frontini; Imbriano, Carol; I., Manni; Mantovani, Roberto. - In: CELL CYCLE. - ISSN 1538-4101. - STAMPA. - 3:(2004), pp. 217-222.

Cell cycle regulation of NF-YC nuclear localization

IMBRIANO, Carol;MANTOVANI, Roberto
2004

Abstract

NF-Y is a trimeric activator with histone fold - HFM - subunits that binds to the CCAAT-box and is required for a majority of cell cycle promoters, often in conjuction with E2Fs. In vivo binding of NF-Y is dynamic during the cell cycle and correlates with gene activation. We performed immunofluorescence studies on endogenous, GFP- and Flag-tagged overexpressed NF-Y subunits. NF-YA, NF-YB are nuclear proteins. Unexpectedly, NF-YC localizes both in cytoplamatic and nuclear compartments and its nuclear localization is determined by the interaction with its heterodimerization partner NF-YB. Most importantly, compartmentalization is regulated during the cell cycle of serum restimulated NIH3T3 cells, accumulating in the nucleus at the onset of S phase. These data point to the control of HFM heterodimerization as an important layer of NF-Y regulation during cell cycle progression.
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Cell cycle regulation of NF-YC nuclear localization / M., Frontini; Imbriano, Carol; I., Manni; Mantovani, Roberto. - In: CELL CYCLE. - ISSN 1538-4101. - STAMPA. - 3:(2004), pp. 217-222.
M., Frontini; Imbriano, Carol; I., Manni; Mantovani, Roberto
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/306776
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