Antagonistic and reciprocal interactions are known to exist between adenosine and dopamine receptors in the striatum. In the present study, double immunofluorescence experiments with confocal laser microscopy showed a high degree of colocalization of adenosine A A receptors (A(2A)R) and dopamine D-2 receptors (D2R) in cell membranes of SH-SY5Y human neuroblastoma cells stably transfected with human D2R and in cultured striatal cells. A(2A)R/D2R heteromeric complexes were demonstrated in coimmunoprecipitation experiments in membrane preparations from D2R-transfected SH-SY5Y cells and from mouse fibroblast Ltk(-) cells stably transfected with human D2R (long form) and transiently cotransfected with the A(2A)R double-tagged with hemagglutinin. Long term exposure to A(2A)R and D2R agonists in D2R-cotransfected SH-SY5Y cells resulted in coaggregation, cointernalization and codesensitization of A(2A)R and D2R. These results give a molecular basis for adenosine-dopamine antagonism at the membrane level and have implications for treatment of Parkinson's disease and schizophrenia, in which D2R are involved.
|Anno di pubblicazione:||2002|
|Titolo:||Coaggregation, cointernalization, and codesensitization of adenosine A(2A) receptors and dopamine D-2 receptors|
|Autore/i:||J. Hillion; M. Canals; M. Torvinen; V. Casado; R. Scott; A. Terasmaa; A. Hansson; S. Watson; ME Olah; J. Mallol; EI Canela; M. Zoli; LF Agnati; CF Ibanez; C. Lluis; R. Franco; S. Ferre; K. Fuxe|
|Codice identificativo ISI:||WOS:000175685100089|
|Codice identificativo Scopus:||2-s2.0-0037124032|
|Tipologia||Articolo su rivista|
File in questo prodotto:
I documenti presenti in Iris Unimore sono rilasciati con licenza Creative Commons Attribuzione - Non commerciale - Non opere derivate 3.0 Italia, salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris