The oral administration of peptidic drugs requires their protection from degradation in the gastric environment and the improvement of their absorption in the intestinal tract. For these requirements, a microsystem based on cross-lined alginate as the carrier of bovine serum albumin (BSA), used as a model protein, was proposed. A spray-drying technique was applied to BSA/sodium alginate solutions to obtain spherical particles having a mean diameter less than 10 mum. the microparticles were hardened using first a solution of calcium chloride and then a solution of chitosan (CS) to obtain stable microsystems. The cross-linking process was carried out at different CS concentrations and pH values of the cross-linking medium. The CS concentration affected the BSA loading in the micro particles prepared at a pH value less than the protein isoelectric point (pI). Moreover, the BSA loading at a pH value less than the pI was higher than that at a pH similar to the pI regardless of the CS concentration. This finding could be attributable to the formation of a BSA/alginate complex. The evaluation of the interaction between BSA and alginate at different pH values by means rheological measurements confirmed this hypothesis. This approach may represent a promising way to devise a microcarrier system with appropriate size for targeting the Peyer's patches, with appropriate immobilization capacity, and suitable for the oral administration of peptidic drugs.
|Data di pubblicazione:||2001|
|Titolo:||Chitosan-alginate microparticles as a protein carrier|
|Autore/i:||Coppi, Gilberto; Iannuccelli, Valentina; Leo, Eliana Grazia; Bernabei, Maria Teresa; Cameroni, Riccardo|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1081/DDC-100104314|
|Codice identificativo ISI:||WOS:000170005100003|
|Codice identificativo Scopus:||2-s2.0-0034952922|
|Codice identificativo Pubmed:||11448046|
|Citazione:||Chitosan-alginate microparticles as a protein carrier / Coppi, Gilberto; Iannuccelli, Valentina; Leo, Eliana Grazia; Bernabei, Maria Teresa; Cameroni, Riccardo. - In: DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY. - ISSN 0363-9045. - STAMPA. - 27(2001), pp. 393-400.|
|Tipologia||Articolo su rivista|
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