A fragment of a putative L-type Ca2+ channel has been identified by molecular biology experiments in immunocytes from the mussel Mytilus galloprovincialis. Using the cell permeable and Ca2+-specific fluorochrome FURA 2-AM, we have demonstrated that the algal toxin yessotoxin (YTX) is able to increase intracellular Ca2+ concentration in M. galloprovincialis immunocytes. The YTX effect on Ca2+ increase is inhibited by the L-type Ca2+ channel inhibitor, verapamil, which is cAMP- and cGMP-dependent; but PKA- and nitric oxide-independent. On the basis of these observations, a possible role for YTX as a potential disturber of mussel immune efficiency is suggested. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Algal toxin yessotoxin signalling pathways involve immunocyte mussel calcium channels / Malagoli, Davide; Casarini, Livio; Ottaviani, Enzo. - In: CELL BIOLOGY INTERNATIONAL. - ISSN 1065-6995. - STAMPA. - 30:9(2006), pp. 721-726. [10.1016/j.cellbi.2006.05.003]
Algal toxin yessotoxin signalling pathways involve immunocyte mussel calcium channels
MALAGOLI, Davide;CASARINI, Livio;OTTAVIANI, Enzo
2006
Abstract
A fragment of a putative L-type Ca2+ channel has been identified by molecular biology experiments in immunocytes from the mussel Mytilus galloprovincialis. Using the cell permeable and Ca2+-specific fluorochrome FURA 2-AM, we have demonstrated that the algal toxin yessotoxin (YTX) is able to increase intracellular Ca2+ concentration in M. galloprovincialis immunocytes. The YTX effect on Ca2+ increase is inhibited by the L-type Ca2+ channel inhibitor, verapamil, which is cAMP- and cGMP-dependent; but PKA- and nitric oxide-independent. On the basis of these observations, a possible role for YTX as a potential disturber of mussel immune efficiency is suggested. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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