The drug release from doxorubicin (DXR)-gelatin nanoparticle conjugates was evaluated by means of a dynamic dialysis technique. The study was carried out in absence and in presence of a proteolytic enzyme (trypsin) able to degrade the carrier. In a preliminary study the apparent permeability constant (K-cv) of the drug through the dialysis bag was evaluated in several media. On the basis of this screening, a saline solution (NaCl 0.9%, w/v) resulted appropriate to carry out the dialysis study since, in this medium, the K-cv did not depend on the drug concentration in the donor solution. In absence of the enzyme only a little fraction (from 9 to 13%, w/w of the drug content) was released from nanoparticles. This fraction was considered as the evidence of the free drug fraction. After the addition of trypsin, the diffusion of a further drug fraction was observed. This fraction is probably due to a fraction of the DXR-peptide conjugates characterised by a molecular weight lower than membrane cut-off (3500 Da). (C) 1999 Elsevier Science B.V. All rights reserved.
Dynamic dialysis for the drug release evaluation from doxorubicin-gelatin nanoparticle conjugates / Leo, Eliana Grazia; R., Cameroni; Forni, Flavio. - In: INTERNATIONAL JOURNAL OF PHARMACEUTICS. - ISSN 0378-5173. - STAMPA. - 180(1999), pp. 23-30.
Data di pubblicazione: | 1999 |
Titolo: | Dynamic dialysis for the drug release evaluation from doxorubicin-gelatin nanoparticle conjugates |
Autore/i: | Leo, Eliana Grazia; R., Cameroni; Forni, Flavio |
Autore/i UNIMORE: | |
Rivista: | |
Volume: | 180 |
Pagina iniziale: | 23 |
Pagina finale: | 30 |
Codice identificativo ISI: | WOS:000079645800003 |
Codice identificativo Scopus: | 2-s2.0-0032998057 |
Codice identificativo Pubmed: | 10089288 |
Citazione: | Dynamic dialysis for the drug release evaluation from doxorubicin-gelatin nanoparticle conjugates / Leo, Eliana Grazia; R., Cameroni; Forni, Flavio. - In: INTERNATIONAL JOURNAL OF PHARMACEUTICS. - ISSN 0378-5173. - STAMPA. - 180(1999), pp. 23-30. |
Tipologia | Articolo su rivista |
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