Free cytosolic [Mg2+] can be assessed in vivo by P-31 MRS from the chemical shift of beta-ATP which in turn depends on the fraction of total ATP complexed to Mg2+ ions, The reliability of these in vive measurements depends on the availability of an appropriate in vitro calibration to determine the limits of chemical shifts of unbound ATP and Mg-ATP complexes, using solutions that mimic the in vive cytosolic conditions as far as possible, We used an algorithm and software to allow a quantitative definition of the Mg2+-binding molecules to build a semi-empirical equation that correlates the chemical shift of the beta-ATP signal to the [Mg2+] taking into account the amount of Mg2+ bound to all other constituents in solution, Our experiments resulted in a simple and reliable equation directly usable to assess in vive the free cytosolic magnesium concentration of human brain by P-31 MRS. Our method is also flexible enough to make it suitable for in vive measurements of [Mg2+] in other organs and tissues.

In vivo assessment of free magnesium concentration in human brain by P-31 MRS. A new calibration curve based on a mathematical algorithm / S., Iotti; Frassineti, Chiara; L., Alderighi; A., Sabatini; A., Vacca; B., Barbiroli. - In: NMR IN BIOMEDICINE. - ISSN 0952-3480. - STAMPA. - 9(1996), pp. 24-32.

In vivo assessment of free magnesium concentration in human brain by P-31 MRS. A new calibration curve based on a mathematical algorithm

FRASSINETI, Chiara;
1996

Abstract

Free cytosolic [Mg2+] can be assessed in vivo by P-31 MRS from the chemical shift of beta-ATP which in turn depends on the fraction of total ATP complexed to Mg2+ ions, The reliability of these in vive measurements depends on the availability of an appropriate in vitro calibration to determine the limits of chemical shifts of unbound ATP and Mg-ATP complexes, using solutions that mimic the in vive cytosolic conditions as far as possible, We used an algorithm and software to allow a quantitative definition of the Mg2+-binding molecules to build a semi-empirical equation that correlates the chemical shift of the beta-ATP signal to the [Mg2+] taking into account the amount of Mg2+ bound to all other constituents in solution, Our experiments resulted in a simple and reliable equation directly usable to assess in vive the free cytosolic magnesium concentration of human brain by P-31 MRS. Our method is also flexible enough to make it suitable for in vive measurements of [Mg2+] in other organs and tissues.
9
24
32
In vivo assessment of free magnesium concentration in human brain by P-31 MRS. A new calibration curve based on a mathematical algorithm / S., Iotti; Frassineti, Chiara; L., Alderighi; A., Sabatini; A., Vacca; B., Barbiroli. - In: NMR IN BIOMEDICINE. - ISSN 0952-3480. - STAMPA. - 9(1996), pp. 24-32.
S., Iotti; Frassineti, Chiara; L., Alderighi; A., Sabatini; A., Vacca; B., Barbiroli
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11380/306300
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