Confocal microscopy reveals that the anti-Bcl-2 antibody (pAb) is able to diffuse across the plasma membrane of the fat body cell line IPLB-LdFB from the insect Lymantria dispar demonstrating the presence of Bcl-2-like molecules in the cytoplasm. Immunoperoxidase procedure confirms the cellular localization. Furthermore, an immunoprecipitation corresponding to a molecular weight of 29 KDa is observed with western blot analysis using the anti-Bcl-2 pAb. Cytofluorimetric experiments show that anti-Bcl-2 pAb counteracts 2-deoxy-D-ribose-induced apoptosis and provokes morphological changes in the insect cell line, i.e. a reduction in cell size, the disappearance of the vacuola and changes in shape. At the same time, the antibody provokes mitochondrial membrane depolarization, and N-acetyl-L-cysteine is unable to reconstitute the physiological conditions. The present findings suggest that Bcl-2-like proteins play a main role in maintaining of the integrity of cellular components, e.g, mitochondria, rather than in controlling programmed cell death.

An anti-Bcl-2 antibody prevents 2-deoxy-D-ribose-induced apoptosis in the IPLB-LdFB insect cell line / D., Barbieri; Malagoli, Davide; B., Cuoghi; Ottaviani, Enzo. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - STAMPA. - 58:(2001), pp. 653-659. [10.1007/PL00000888]

An anti-Bcl-2 antibody prevents 2-deoxy-D-ribose-induced apoptosis in the IPLB-LdFB insect cell line

MALAGOLI, Davide;OTTAVIANI, Enzo
2001

Abstract

Confocal microscopy reveals that the anti-Bcl-2 antibody (pAb) is able to diffuse across the plasma membrane of the fat body cell line IPLB-LdFB from the insect Lymantria dispar demonstrating the presence of Bcl-2-like molecules in the cytoplasm. Immunoperoxidase procedure confirms the cellular localization. Furthermore, an immunoprecipitation corresponding to a molecular weight of 29 KDa is observed with western blot analysis using the anti-Bcl-2 pAb. Cytofluorimetric experiments show that anti-Bcl-2 pAb counteracts 2-deoxy-D-ribose-induced apoptosis and provokes morphological changes in the insect cell line, i.e. a reduction in cell size, the disappearance of the vacuola and changes in shape. At the same time, the antibody provokes mitochondrial membrane depolarization, and N-acetyl-L-cysteine is unable to reconstitute the physiological conditions. The present findings suggest that Bcl-2-like proteins play a main role in maintaining of the integrity of cellular components, e.g, mitochondria, rather than in controlling programmed cell death.
2001
58
653
659
An anti-Bcl-2 antibody prevents 2-deoxy-D-ribose-induced apoptosis in the IPLB-LdFB insect cell line / D., Barbieri; Malagoli, Davide; B., Cuoghi; Ottaviani, Enzo. - In: CELLULAR AND MOLECULAR LIFE SCIENCES. - ISSN 1420-682X. - STAMPA. - 58:(2001), pp. 653-659. [10.1007/PL00000888]
D., Barbieri; Malagoli, Davide; B., Cuoghi; Ottaviani, Enzo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/305512
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