We assessed the functional properties and the kinetic status in vitro, and the engraftment potential in vivo of human haematopoietic stem cells according to the expression of CD34 antigen. Lin(-)CD34(-) and Lin(-)CD34(+) cells were isolated from granulocyte colony-stimulating factor-primed peripheral blood (PB) cells of healthy donors. The CD34(-) cell fraction did not contain either clonogenic cells in semisolid culture or long-term culture initiating cells (LTC-IC). However, stroma-dependent liquid cultures and cytokines induced CD34 expression on a minority of stem cells, acquisition of clonogenic capacity and generation of LTC-IC. Significantly higher percentages of quiescent G(0) cells and lower percentages of cycling G(1) cells were found in Lin(-)CD34(-) cells when compared with Lin(-)CD34(+) cells. Kinetic quiescence of Lin(-)CD34(-) cells was associated with a significantly higher expression of the negative regulators of the cell cycle, p27(Kip1) and p21(cip1/waf1). Cytokine-mediated induction of CD34, in vitro, resulted in cycling of stem cells and downregulation of p27. There was a higher rate of human long-term engraftment in immunocompromised non-obese diabetic (NOD)/recombination activating gene 1(null) and NOD/severe combined immunodeficient-beta(2)microglobulin(null) mice injected with CD34(+) cells. Thus, our study indicated that CD34 expression on human PB stem cells was associated with haematopoietic activity, cell-cycle recruitment and downregulation of p27(Kip1)in vitro and higher engraftment capacity in vivo.

Functional and kinetic characterization of granulocyte colony-stimulating factor-primed CD34) human stem cells / Rm, Lemoli; F., Bertolini; Mt, Petrucci; C., Gregorj; Mr, Ricciardi; M., Fogli; A., Curti; C., Rabascio; S., Pandolfi; Ferrari, Sergio; R., Fo; M., Baccarani; A., Tafuri. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - 123:(2003), pp. 720-729.

Functional and kinetic characterization of granulocyte colony-stimulating factor-primed CD34) human stem cells

FERRARI, Sergio;
2003

Abstract

We assessed the functional properties and the kinetic status in vitro, and the engraftment potential in vivo of human haematopoietic stem cells according to the expression of CD34 antigen. Lin(-)CD34(-) and Lin(-)CD34(+) cells were isolated from granulocyte colony-stimulating factor-primed peripheral blood (PB) cells of healthy donors. The CD34(-) cell fraction did not contain either clonogenic cells in semisolid culture or long-term culture initiating cells (LTC-IC). However, stroma-dependent liquid cultures and cytokines induced CD34 expression on a minority of stem cells, acquisition of clonogenic capacity and generation of LTC-IC. Significantly higher percentages of quiescent G(0) cells and lower percentages of cycling G(1) cells were found in Lin(-)CD34(-) cells when compared with Lin(-)CD34(+) cells. Kinetic quiescence of Lin(-)CD34(-) cells was associated with a significantly higher expression of the negative regulators of the cell cycle, p27(Kip1) and p21(cip1/waf1). Cytokine-mediated induction of CD34, in vitro, resulted in cycling of stem cells and downregulation of p27. There was a higher rate of human long-term engraftment in immunocompromised non-obese diabetic (NOD)/recombination activating gene 1(null) and NOD/severe combined immunodeficient-beta(2)microglobulin(null) mice injected with CD34(+) cells. Thus, our study indicated that CD34 expression on human PB stem cells was associated with haematopoietic activity, cell-cycle recruitment and downregulation of p27(Kip1)in vitro and higher engraftment capacity in vivo.
123
720
729
Functional and kinetic characterization of granulocyte colony-stimulating factor-primed CD34) human stem cells / Rm, Lemoli; F., Bertolini; Mt, Petrucci; C., Gregorj; Mr, Ricciardi; M., Fogli; A., Curti; C., Rabascio; S., Pandolfi; Ferrari, Sergio; R., Fo; M., Baccarani; A., Tafuri. - In: BRITISH JOURNAL OF HAEMATOLOGY. - ISSN 0007-1048. - 123:(2003), pp. 720-729.
Rm, Lemoli; F., Bertolini; Mt, Petrucci; C., Gregorj; Mr, Ricciardi; M., Fogli; A., Curti; C., Rabascio; S., Pandolfi; Ferrari, Sergio; R., Fo; M., Baccarani; A., Tafuri
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/305120
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