Blue copper proteins are type-I copper containing redox proteins whose role is to shuttle electrons from an electron donor to an electron acceptor in bacteria and plants. A large amount of experimental data is available on blue copper proteins; however, their functional characterization is hindered by the complexity of redox processes in biological systems. We describe here the application of a semiquantitative method based on a comparative analysis of molecular interaction fields to gain insights into the recognition properties of blue copper proteins. Molecular electrostatic and hydrophobic potentials were computed and compared for a set of 33 experimentally-determined structures of proteins from seven blue copper subfamilies, and the results were quantified by means of similarity indices. The analysis provides a classification of the blue copper proteins and shows that (1) comparison of the molecular electrostatic potentials provides useful information complementary to that highlighted by sequence analysis; (2) similarities in recognition properties can be detected for proteins belonging to different subfamilies, such as amicyanins and pseudoazurins, that may be isofunctional proteins; (3) dissimilarities in interaction properties, consistent with experimentally different binding specificities, may be observed between proteins belonging to the same subfamily, such as cyanobacterial and eukaryotic plastocyanins; (4) proteins with low sequence identity, such as azurins and pseudoazurins, can have sufficient similarity to bind to similar electron donors and accepters while having different binding specificity profiles.
|Data di pubblicazione:||2000|
|Titolo:||Blue copper proteins: A comparative analysis of their molecular interaction properties|
|Autore/i:||DE RIENZO, Francesca; Rr, Gabdoulline; Menziani, Maria Cristina; Rc, Wade|
|Codice identificativo ISI:||WOS:000088917400003|
|Codice identificativo Scopus:||2-s2.0-0033841688|
|Codice identificativo Pubmed:||10975566|
|Citazione:||Blue copper proteins: A comparative analysis of their molecular interaction properties / DE RIENZO, Francesca; Rr, Gabdoulline; Menziani, Maria Cristina; Rc, Wade. - In: PROTEIN SCIENCE. - ISSN 0961-8368. - STAMPA. - 9(2000), pp. 1439-1454.|
|Tipologia||Articolo su rivista|
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