Objective: In chronically hypertensive (CH), preeclamptic (PE), and normotensive pregnant women (N), we investigated ex vivo platelet aggregation in response to L-arginine (L-Arg) and sodium nitroprusside (SN), which are respectively the substrate and donor of nitric oxide (NO). Methods: Platelet aggregation was determined with a dual-channel aggregometer by measuring transmittance of light through the sample in comparison to platelet poor plasma, as a reference. Aggregation induced by adenosine diphosphate was continuously recorded for 3 min and measured before and after preincubation with L-Arg and SN. Results: Preincubation with L-Arg significantly reduced platelet aggregation in N and CH patients (p < 0.05) but not in PE women. Preincubation with SN affected aggregation in PE women also (p < 0.001). No correlation was found between platelet response to L-Arg or SN stimuli and the severity of hypertensive disorders expressed as week of gestation at delivery or birth weight. Conclusions: The present study demonstrates that a decreased platelet sensitivity to L-Arg characterizes PE women, whereas SN maintains its antithrombotic power. This impairment seems to be specific for PE, because platelets of CH patients utilize L-Arg normally. This finding supports the involvement of the L-Arg-NO pathway in the pathogenesis of the procoagulative features of PE and probably in the onset of the disease. The maintained response to SN in PE patients suggests a possible therapeutical use of NO donors in the disease.
|Data di pubblicazione:||2000|
|Titolo:||Platelet responsiveness to L-arginine in hypertensive disorders of pregnancy|
|Autore/i:||Neri, Isabella; F., Piccinini; M., Marietta; Facchinetti, Fabio; Volpe, Annibale|
|Codice identificativo ISI:||WOS:000165449900009|
|Codice identificativo Scopus:||2-s2.0-0033677653|
|Codice identificativo Pubmed:||11118406|
|Citazione:||Platelet responsiveness to L-arginine in hypertensive disorders of pregnancy / Neri, Isabella; F., Piccinini; M., Marietta; Facchinetti, Fabio; Volpe, Annibale. - In: HYPERTENSION IN PREGNANCY. - ISSN 1064-1955. - STAMPA. - 19(2000), pp. 323-330.|
|Tipologia||Articolo su rivista|
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