Three natural chondroitin sulfates (CSs), from porcine and bovine trachea, and from shark cartilage, were studied using a variety of NMR techniques (DQS, TOCSY, NOESY, HMQC). A good H-1 and C-13 characterization of the major components, chondroitin 4-sulfate (CS4) and chondroitin 6-sulfate (CS6), was obtained and a number of signals coming from chondroitin 2,6-disulfate (CS2,6) (present only in shark CS) was identified. The study of a chemically desulfated CS was necessary in order to understand the difficulties encountered in detecting signals from the chondroitin non-sulfate (CS0) component of porcine and bovine CSs. The singular pattern of UC-4 and NC-1 signals was recognized and explained in terms of a diad model. The excess of multiplicity affecting mainly these two signals was attributed to the differences in the conformation of the N 1:4 U glycosidic bond. Further support to this hypothesis comes from the comparison of the NOESY spectra of the three CSs. (C) 2000 Elsevier Science Ltd. All rights reserved.

H-1 and C-13 nuclear magnetic resonance identification and characterization of components of chondroitin sulfates of various origin / Mucci, Adele; Schenetti, Luisa; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 41:(2000), pp. 37-45.

H-1 and C-13 nuclear magnetic resonance identification and characterization of components of chondroitin sulfates of various origin

MUCCI, Adele;SCHENETTI, Luisa;VOLPI, Nicola
2000-01-01

Abstract

Three natural chondroitin sulfates (CSs), from porcine and bovine trachea, and from shark cartilage, were studied using a variety of NMR techniques (DQS, TOCSY, NOESY, HMQC). A good H-1 and C-13 characterization of the major components, chondroitin 4-sulfate (CS4) and chondroitin 6-sulfate (CS6), was obtained and a number of signals coming from chondroitin 2,6-disulfate (CS2,6) (present only in shark CS) was identified. The study of a chemically desulfated CS was necessary in order to understand the difficulties encountered in detecting signals from the chondroitin non-sulfate (CS0) component of porcine and bovine CSs. The singular pattern of UC-4 and NC-1 signals was recognized and explained in terms of a diad model. The excess of multiplicity affecting mainly these two signals was attributed to the differences in the conformation of the N 1:4 U glycosidic bond. Further support to this hypothesis comes from the comparison of the NOESY spectra of the three CSs. (C) 2000 Elsevier Science Ltd. All rights reserved.
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H-1 and C-13 nuclear magnetic resonance identification and characterization of components of chondroitin sulfates of various origin / Mucci, Adele; Schenetti, Luisa; Volpi, Nicola. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - STAMPA. - 41:(2000), pp. 37-45.
Mucci, Adele; Schenetti, Luisa; Volpi, Nicola
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/304383
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