In this study, we used in vitro electrophysiology along with immunohistochemistry and molecular techniques to study the subiculum-a limbic structure that gates the information flow from and to the hippocampus-in pilocarpine-treated epileptic rats. Comparative data were obtained from age-matched nonepileptic controls (NEC). Subicular neurons in hippocampal-entorhinal cortex (EC) slices of epileptic rats were: (i) hyperexcitable when activated by CA1 or EC inputs; and (ii) generated spontaneous postsynaptic potentials at higher frequencies than NEC cells. Analysis of pharmacologically isolated, GABA(A) receptor-mediated inhibitory postsynaptic potentials revealed more positive reversal potentials in epileptic tissue (-67.8 +/- 6.3 mV, n = 16 vs. -74.8 +/- 3.6 mV in NEC, n = 13; P < 0.001) combined with a reduction in peak conductance (17.6 +/- 11.3 nS vs. 41.1 +/- 26.7 nS in NEC; P < 0.003). These electrophysiological data correlated in the epileptic subiculum with (i) reduced levels of mRNA expression and immunoreactivity of the neuron-specific potassium-chloride cotransporter 2; (ii) decreased number of parvalbumin-positive cells; and (iii) increased synaptophysin (a putative marker of sprouting) immunoreactivity. These findings identify an increase in network excitability within the subiculum of pilocarpine-treated, epileptic rats and point at a reduction in inhibition as an underlying mechanism. (c) 2006 Wiley-Liss, Inc.

Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy / P., de Guzman; Y., Inaba; Biagini, Giuseppe; Baldelli, Enrica; C., Mollinari; D., Merlo; M., Avoli. - In: HIPPOCAMPUS. - ISSN 1050-9631. - STAMPA. - 16:10(2006), pp. 843-860. [10.1002/hipo.20215]

Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy

BIAGINI, Giuseppe;BALDELLI, Enrica;
2006

Abstract

In this study, we used in vitro electrophysiology along with immunohistochemistry and molecular techniques to study the subiculum-a limbic structure that gates the information flow from and to the hippocampus-in pilocarpine-treated epileptic rats. Comparative data were obtained from age-matched nonepileptic controls (NEC). Subicular neurons in hippocampal-entorhinal cortex (EC) slices of epileptic rats were: (i) hyperexcitable when activated by CA1 or EC inputs; and (ii) generated spontaneous postsynaptic potentials at higher frequencies than NEC cells. Analysis of pharmacologically isolated, GABA(A) receptor-mediated inhibitory postsynaptic potentials revealed more positive reversal potentials in epileptic tissue (-67.8 +/- 6.3 mV, n = 16 vs. -74.8 +/- 3.6 mV in NEC, n = 13; P < 0.001) combined with a reduction in peak conductance (17.6 +/- 11.3 nS vs. 41.1 +/- 26.7 nS in NEC; P < 0.003). These electrophysiological data correlated in the epileptic subiculum with (i) reduced levels of mRNA expression and immunoreactivity of the neuron-specific potassium-chloride cotransporter 2; (ii) decreased number of parvalbumin-positive cells; and (iii) increased synaptophysin (a putative marker of sprouting) immunoreactivity. These findings identify an increase in network excitability within the subiculum of pilocarpine-treated, epileptic rats and point at a reduction in inhibition as an underlying mechanism. (c) 2006 Wiley-Liss, Inc.
2006
16
10
843
860
Subiculum network excitability is increased in a rodent model of temporal lobe epilepsy / P., de Guzman; Y., Inaba; Biagini, Giuseppe; Baldelli, Enrica; C., Mollinari; D., Merlo; M., Avoli. - In: HIPPOCAMPUS. - ISSN 1050-9631. - STAMPA. - 16:10(2006), pp. 843-860. [10.1002/hipo.20215]
P., de Guzman; Y., Inaba; Biagini, Giuseppe; Baldelli, Enrica; C., Mollinari; D., Merlo; M., Avoli
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/304215
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