Background: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There ir a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms. Materials and Methods. (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancers from 106 patients. Results: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence. Conclusions: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer.

Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma / G., Romano; L., Garagnani; A., Boninsegna; Ferrari, Paola; G., Flamini; DE GAETANI, Carmela; A., Sgambato; F., Giovanni; G., Curigliano; G., Ferretti; A., Cittadini; Trentini, Giampaolo. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 19:(1999), pp. 4571-4576.

Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma

FERRARI, Paola;DE GAETANI, Carmela;TRENTINI, Giampaolo
1999

Abstract

Background: Activated intermediates of 4-aminobiphenyl (4-ABP) are able to covalently interact with DNA to form adducts. There ir a large body of evidence indicating that carcinogen-DNA adduct formation can be one of the cancer initiating mechanisms. Materials and Methods. (4-ABP)-induced DNA damage in association with p53 overexpression and mutations were evaluated in specimens of urothelial bladder cancers from 106 patients. Results: 4-ABP-DNA adduct levels resulted higher in smokers compared to non smokers, with a borderline statistical value. p53 nuclear overexpression was related to tumor grading, while no significant correlation with stage, 4-ABP-DNA adducts, smoking habit, and disease recurrence could be observed. Concerning molecular analysis, p53 point mutations were found in 17 of 106 cases (16%) and mutational pattern was significantly associated both with higher grade and stage, but no correlation was found with disease recurrence. Conclusions: These results suggest that other sources, in addition to tobacco smoke, may contribute to 4-ABP-DNA adducts formation in bladder tissue and that p53 expression/mutation cannot be considered a prognostic factor in bladder cancer.
19
4571
4576
Analysis of 4-ABP-DNA adducts and p53 alterations in urinary bladder carcinoma / G., Romano; L., Garagnani; A., Boninsegna; Ferrari, Paola; G., Flamini; DE GAETANI, Carmela; A., Sgambato; F., Giovanni; G., Curigliano; G., Ferretti; A., Cittadini; Trentini, Giampaolo. - In: ANTICANCER RESEARCH. - ISSN 0250-7005. - 19:(1999), pp. 4571-4576.
G., Romano; L., Garagnani; A., Boninsegna; Ferrari, Paola; G., Flamini; DE GAETANI, Carmela; A., Sgambato; F., Giovanni; G., Curigliano; G., Ferretti; A., Cittadini; Trentini, Giampaolo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/303798
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