The operational equivalence of different types of tumor promoters was studied by comparing immediate, early, and late effects of okadaic acid (OA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the phosphorylation state of p42 mitogen activated protein kinase isoform (ERK2) in eight different cell lines. In normal human and mouse fibroblasts, both agents stimulated immediate/early (15-60 min) phosphorylation of ERK2. In mouse 3T3 cells, enhanced phosphorylation of ERK2 was detected only within the first hour of treatment with TPA but not with OA. The early response to both TPA and OA, in rum, was lost in another established cell line, the PNT2 prostate epithelial cells, where we could detect increased levels of phosphorylated ERK2 only after a 24-hr treatment with OA. When the effect of OA was evaluated in different PNT cell strains, we observed that their capacity to respond to this agent, by stabilizing phosphorylated forms of ERK2, was lost in less differentiated strains. In HeLa S-3 and HTC tumor cells, however, neither TPA nor OA treatment led to any detectable increase in ERK2 phosphorylation at any time point analyzed. We conclude that the effects of OA and TPA on the phosphorylation states of ERK2 could be related to the cell type, and that the operational equivalence between these two different tumor promoters is maximal in normal cells.

Different sensitivities of p42 mitogen-activated protein kinase to phorbol ester and okadaic acid tumor promoters among cell types / Rossini, Gian Paolo; C., Pinna; C., Malaguti. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - STAMPA. - 58:(1999), pp. 279-284.

Different sensitivities of p42 mitogen-activated protein kinase to phorbol ester and okadaic acid tumor promoters among cell types

ROSSINI, Gian Paolo;
1999

Abstract

The operational equivalence of different types of tumor promoters was studied by comparing immediate, early, and late effects of okadaic acid (OA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the phosphorylation state of p42 mitogen activated protein kinase isoform (ERK2) in eight different cell lines. In normal human and mouse fibroblasts, both agents stimulated immediate/early (15-60 min) phosphorylation of ERK2. In mouse 3T3 cells, enhanced phosphorylation of ERK2 was detected only within the first hour of treatment with TPA but not with OA. The early response to both TPA and OA, in rum, was lost in another established cell line, the PNT2 prostate epithelial cells, where we could detect increased levels of phosphorylated ERK2 only after a 24-hr treatment with OA. When the effect of OA was evaluated in different PNT cell strains, we observed that their capacity to respond to this agent, by stabilizing phosphorylated forms of ERK2, was lost in less differentiated strains. In HeLa S-3 and HTC tumor cells, however, neither TPA nor OA treatment led to any detectable increase in ERK2 phosphorylation at any time point analyzed. We conclude that the effects of OA and TPA on the phosphorylation states of ERK2 could be related to the cell type, and that the operational equivalence between these two different tumor promoters is maximal in normal cells.
1999
58
279
284
Different sensitivities of p42 mitogen-activated protein kinase to phorbol ester and okadaic acid tumor promoters among cell types / Rossini, Gian Paolo; C., Pinna; C., Malaguti. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - STAMPA. - 58:(1999), pp. 279-284.
Rossini, Gian Paolo; C., Pinna; C., Malaguti
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/303654
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 13
  • ???jsp.display-item.citation.isi??? 11
social impact