We have monitored viscoelasticity variation of a cell population during the cell cycle by a Quartz Crystal Microbalance (QCM). Balb 3T3 fibroblasts were synchronized in the G0/G1 phase and seeded in a QCM chamber placed in a cell incubator. After cell sedimentation, the frequency signal was characterized by an amplitude modulation attributed to the viscoelasticity variation of the cells proliferating in phase. A control experiment with nonsynchronized cells showed a similar signal trend, but without significant modulation. Interestingly, the system resulted also to perform as a device sensitive to the effect of drugs affecting the cell cycle, such as colchicine.
Monitoring cell-cycle-related viscoelasticity by a quartz crystal microbalance / Alessandrini, Andrea; Croce, Maria Antonietta; Tiozzo, Roberta; Facci, P.. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - STAMPA. - 88:(2006), pp. 083905-1-083905-3. [10.1063/1.2177647]
Monitoring cell-cycle-related viscoelasticity by a quartz crystal microbalance
ALESSANDRINI, Andrea;CROCE, Maria Antonietta;TIOZZO, Roberta;
2006
Abstract
We have monitored viscoelasticity variation of a cell population during the cell cycle by a Quartz Crystal Microbalance (QCM). Balb 3T3 fibroblasts were synchronized in the G0/G1 phase and seeded in a QCM chamber placed in a cell incubator. After cell sedimentation, the frequency signal was characterized by an amplitude modulation attributed to the viscoelasticity variation of the cells proliferating in phase. A control experiment with nonsynchronized cells showed a similar signal trend, but without significant modulation. Interestingly, the system resulted also to perform as a device sensitive to the effect of drugs affecting the cell cycle, such as colchicine.
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