In the present study, somatic metaphase chromosomes of the millipede Enologus oxypygum (Diplopoda: Julida) were hybridized in situ with a sea urchin (Echinodermata) ribosomal probe (prR14) in order to map major RNA genes (rDNA). Chromosomal preparations were also silver stained (Ag-NOR) to evaluate the rDNA transcriptional activity. Our results indicate that RNA genes are throughout heterochromatin in eight chromosomes involving 1/4 of the total heterochromatin which, in this species, is about 67% of the total DNA. Ag-NOR and FISH patterns were not coincident.
18S-28S rDNA is interspersed throughout heterochromatin in the millepede Enologus oxypygum (Diplopoda) / R., Vitturi; M. S., Colomba; Mandrioli, Mauro; A. M., Pirrone; G., Grispo; R. Barbieri, R.. - In: GENETICA. - ISSN 0016-6707. - STAMPA. - 110:(2000), pp. 151-155. [10.1023/A:1017928826304]
18S-28S rDNA is interspersed throughout heterochromatin in the millepede Enologus oxypygum (Diplopoda).
MANDRIOLI, Mauro;
2000
Abstract
In the present study, somatic metaphase chromosomes of the millipede Enologus oxypygum (Diplopoda: Julida) were hybridized in situ with a sea urchin (Echinodermata) ribosomal probe (prR14) in order to map major RNA genes (rDNA). Chromosomal preparations were also silver stained (Ag-NOR) to evaluate the rDNA transcriptional activity. Our results indicate that RNA genes are throughout heterochromatin in eight chromosomes involving 1/4 of the total heterochromatin which, in this species, is about 67% of the total DNA. Ag-NOR and FISH patterns were not coincident.
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