Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes—5S rDNA, 18S–26S rDNA, and (TTAGGG)n. Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S–26S rDNA as probes. Simultaneous (18S–26S)-5S and (18S–26S)-(TTAGGG)nFISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.
rDNA (18S-28S and 5S) co-localization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae) revealed by single- and double-colour FISH / R., Vitturi; M. S., Colomba; A. M., Pirrone; Mandrioli, Mauro. - In: JOURNAL OF HEREDITY. - ISSN 0022-1503. - STAMPA. - 93:4(2002), pp. 279-282. [10.1093/jhered/93.4.279]
rDNA (18S-28S and 5S) co-localization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae) revealed by single- and double-colour FISH
MANDRIOLI, Mauro
2002
Abstract
Spermatogonial and metaphase I chromosomes of the lumbricid earthworm Octodrilus complanatus (Annelida: Oligochaeta) were examined using fluorescent in situ hybridization (FISH) with three repetitive DNA probes—5S rDNA, 18S–26S rDNA, and (TTAGGG)n. Single-color FISH consistently mapped one chromosome pair per spread using either 5S rDNA or 18S–26S rDNA as probes. Simultaneous (18S–26S)-5S and (18S–26S)-(TTAGGG)nFISH demonstrated that repeated units of the two ribosomal families were overlapped and closely associated with telomeric sequences.File | Dimensione | Formato | |
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