Ghrelin is a new gastric peptide involved in food intake control and growth hormone release. We aimed to assess its cell localisation in man during adult and fetal life and to clarify present interspecies inconsistencies of gastric endocrine cell types. A specific serum generated against amino acids 13-28 of ghrelin was tested on fetal and adult gastric mucosa and compared with ghrelin in situ hybridisation. Immunogold electron microscopy was performed on normal human, rat and dog adult stomach. Ghrelin cells were detected in developing gut, pancreas and lung from gestational week 10 and in adult human, rat and dog gastric mucosa. By immunogold electron microscopy, gastric ghrelin cells showed distinctive morphology and hormone reactivity in respect to histamine enterochromaffin-like, somatostatin D, glucagon A or serotonin enterochromaffin cells. Ghrelin cells were characterised by round, compact, electron-dense secretory granules of P/D(1) type in man (mean diameter 147+/-30 nm), A-like type in the rat (183+/-37 nm) and X type in the dog (273+/-49 nm). It is concluded that, ghrelin is produced by well-defined cell types, which in the past had been labelled differently in various mammals mostly because of the different size of their secretory granule. In man ghrelin cells develop during early fetal life.

Characterisation of gastric ghrelin cells in man and other mammals: studies in adult and fetal tissues / Rindi, G.; Necchi, V.; Savio, A.; Torsello, A.; Zoli, Michele; Locatelli, V.; Raimondo, F.; Cocchi, D.; Solcia, E.. - In: HISTOCHEMISTRY AND CELL BIOLOGY. - ISSN 0948-6143. - STAMPA. - 117:(2002), pp. 511-519.

Characterisation of gastric ghrelin cells in man and other mammals: studies in adult and fetal tissues.

ZOLI, Michele;
2002

Abstract

Ghrelin is a new gastric peptide involved in food intake control and growth hormone release. We aimed to assess its cell localisation in man during adult and fetal life and to clarify present interspecies inconsistencies of gastric endocrine cell types. A specific serum generated against amino acids 13-28 of ghrelin was tested on fetal and adult gastric mucosa and compared with ghrelin in situ hybridisation. Immunogold electron microscopy was performed on normal human, rat and dog adult stomach. Ghrelin cells were detected in developing gut, pancreas and lung from gestational week 10 and in adult human, rat and dog gastric mucosa. By immunogold electron microscopy, gastric ghrelin cells showed distinctive morphology and hormone reactivity in respect to histamine enterochromaffin-like, somatostatin D, glucagon A or serotonin enterochromaffin cells. Ghrelin cells were characterised by round, compact, electron-dense secretory granules of P/D(1) type in man (mean diameter 147+/-30 nm), A-like type in the rat (183+/-37 nm) and X type in the dog (273+/-49 nm). It is concluded that, ghrelin is produced by well-defined cell types, which in the past had been labelled differently in various mammals mostly because of the different size of their secretory granule. In man ghrelin cells develop during early fetal life.
117
511
519
Characterisation of gastric ghrelin cells in man and other mammals: studies in adult and fetal tissues / Rindi, G.; Necchi, V.; Savio, A.; Torsello, A.; Zoli, Michele; Locatelli, V.; Raimondo, F.; Cocchi, D.; Solcia, E.. - In: HISTOCHEMISTRY AND CELL BIOLOGY. - ISSN 0948-6143. - STAMPA. - 117:(2002), pp. 511-519.
Rindi, G.; Necchi, V.; Savio, A.; Torsello, A.; Zoli, Michele; Locatelli, V.; Raimondo, F.; Cocchi, D.; Solcia, E.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/20832
Citazioni
  • ???jsp.display-item.citation.pmc??? 43
  • Scopus 198
  • ???jsp.display-item.citation.isi??? 174
social impact