OBJECTIVE: Aim of the study is to assess the presence of spontaneous chromosome damage in patients affected by limited (lSSc) or diffuse (dSSc) Systemic Slerosis, using the micronucleus (MN) assay.METHODS: We evaluated MN frequency in cultured peripheral lymphocytes of 18 SSc and in a group of 20 healthy controls. Patients were also classified as ACA+, Scl70+, FAN+ according to the presence of the specific anti-nuclear antibodies. We also explored the hypothesis that the extent of cytogenetic alteration might be related to the severity of the pathological condition and/or to the immunological profile.RESULTS: Compared to controls, the patient group as a whole showed significantly higher MN frequencies (10.8+/-4.5 vs. 27.8+/-13.7, p<0.001). No correlation was found between spontaneous chromosome damage and severity of the disease, being MN frequency 33.1+/-17.0 and 19.8+/-2.7 in lSSc and dSSc, respectively. Interestingly, ACA+ subjects displayed the highest MN frequency (36.9+/-15.0), as compared to patients with different antibody pattern (Scl70+, FAN+; 19.7+/-8.2).CONCLUSIONS: Our results confirm the presence of chromosomal damage in circulating lymphocytes of SSc patients and would suggest a key role of antibodies to the centromere in determining the observed cytogenetic anomalies.

The high frequency of spontaneous micronuclei observed in lymphocytes of systemic sclerosis patients: preliminary results / Porciello, G.; Scarpato, R.; Storino, F.; Cagetti, F.; Marcolongo, R.; Migliore, L.; Ferri, Clodoveo; Galeazzi, M.. - In: REUMATISMO. - ISSN 0048-7449. - STAMPA. - 54:(2002), pp. 36-39.

The high frequency of spontaneous micronuclei observed in lymphocytes of systemic sclerosis patients: preliminary results.

FERRI, Clodoveo;
2002-01-01

Abstract

OBJECTIVE: Aim of the study is to assess the presence of spontaneous chromosome damage in patients affected by limited (lSSc) or diffuse (dSSc) Systemic Slerosis, using the micronucleus (MN) assay.METHODS: We evaluated MN frequency in cultured peripheral lymphocytes of 18 SSc and in a group of 20 healthy controls. Patients were also classified as ACA+, Scl70+, FAN+ according to the presence of the specific anti-nuclear antibodies. We also explored the hypothesis that the extent of cytogenetic alteration might be related to the severity of the pathological condition and/or to the immunological profile.RESULTS: Compared to controls, the patient group as a whole showed significantly higher MN frequencies (10.8+/-4.5 vs. 27.8+/-13.7, p<0.001). No correlation was found between spontaneous chromosome damage and severity of the disease, being MN frequency 33.1+/-17.0 and 19.8+/-2.7 in lSSc and dSSc, respectively. Interestingly, ACA+ subjects displayed the highest MN frequency (36.9+/-15.0), as compared to patients with different antibody pattern (Scl70+, FAN+; 19.7+/-8.2).CONCLUSIONS: Our results confirm the presence of chromosomal damage in circulating lymphocytes of SSc patients and would suggest a key role of antibodies to the centromere in determining the observed cytogenetic anomalies.
54
36
39
The high frequency of spontaneous micronuclei observed in lymphocytes of systemic sclerosis patients: preliminary results / Porciello, G.; Scarpato, R.; Storino, F.; Cagetti, F.; Marcolongo, R.; Migliore, L.; Ferri, Clodoveo; Galeazzi, M.. - In: REUMATISMO. - ISSN 0048-7449. - STAMPA. - 54:(2002), pp. 36-39.
Porciello, G.; Scarpato, R.; Storino, F.; Cagetti, F.; Marcolongo, R.; Migliore, L.; Ferri, Clodoveo; Galeazzi, M.
File in questo prodotto:
File Dimensione Formato  
55-Article Text-106-1-10-20110315.pdf

Open access

Tipologia: Versione pubblicata dall'editore
Dimensione 34.24 kB
Formato Adobe PDF
34.24 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Licenza Creative Commons
I metadati presenti in IRIS UNIMORE sono rilasciati con licenza Creative Commons CC0 1.0 Universal, mentre i file delle pubblicazioni sono rilasciati con licenza Attribuzione 4.0 Internazionale (CC BY 4.0), salvo diversa indicazione.
In caso di violazione di copyright, contattare Supporto Iris

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11380/20444
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 15
  • ???jsp.display-item.citation.isi??? ND
social impact