Background: Long-term daily use of aspirin reduces incidence and mortality due to colorectal cancer (CRC). This study aimed to analyze the effect of aspirin on the tumor microenvironment, systemic immunity, and on the healthy mucosa surrounding cancer. Methods: Patients with a diagnosis of CRC operated on from 2015 to 2019 were retrospectively analyzed (METACCRE cohort). Expression of mRNA of immune surveillance-related genes (PD-L1, CD80, CD86, HLA I, and HLA II) in CRC primary cells treated with aspirin were extracted from Gene Expression Omnibus–deposited public database (GSE76583). The experiment was replicated in cell lines. The mucosal immune microenvironment of a subgroup of patients participating in the IMMUNOREACT1 (ClinicalTrials.gov NCT04915326) project was analyzed with immunohistochemistry and flow cytometry. Results: In the METACCRE Cohort, 12% of 238 patients analyzed were aspirin users. Nodal metastasis was significantly less frequent (p =.008) and tumor-infiltrating lymphocyte infiltration was higher (p =.02) among aspirin users. In the CRC primary cells and selected cell lines, CD80 mRNA expression was increased following aspirin treatment (p =.001). In the healthy mucosa surrounding rectal cancer, the ratio of CD8/CD3 and epithelial cells expressing CD80 was higher in aspirin users (p =.027 and p =.034, respectively). Conclusions: These data suggested that regular aspirin use may have an active role in enhancing immunosurveillance against CRC.
IMMUNOREACT 7: Regular aspirin use is associated with immune surveillance activation in colorectal cancer / Simoni, Ottavia De; Scarpa, Melania; Castagliuolo, Ignazio; Stepanyan, Astghik; Angriman, Imerio; Kotsafti, Andromachi; Nacci, Camilla; Scognamiglio, Federico; Negro, Silvia; D'Angelo, Antonella; Chiminazzo, Valentina; Businello, Gianluca; Ruffolo, Cesare; Salmaso, Roberta; Franzato, Boris; Gruppo, Mario; Pilati, Pierluigi; Scapinello, Antonio; Pozza, Anna; Stecca, Tommaso; Massani, Marco; Cataldo, Ivana; Brignola, Stefano; Dei Tos, Angelo Paolo; Ceccon, Carlotta; Guzzardo, Vincenza; Vignotto, Chiara; Facci, Luca; Maretto, Isacco; Agostini, Marco; Marchegiani, Francesco; Becherucci, Giulia; Zizzo, Maurizio; Bordignon, Giovanni; Merenda, Roberto; Pirozzolo, Giovanni; Recordare, Alfonso; Pozza, Giulia; Godina, Mario; Mondi, Isabella; Verdi, Daunia; Lio, Corrado Da; Laurino, Licia; Saadeh, Luca; Rivella, Giorgio; Guerriero, Silvio; Romiti, Chiara; Portale, Giuseppe; Cipollari, Chiara; Spolverato, Ylenia Camilla; Noaro, Giulia; Cola, Roberto; Candioli, Salvatore; Gavagna, Laura; Ricagna, Fabio; Ortenzi, Monica; Guerrieri, Mario; Tagliente, Giovanni; Tomassi, Monica; Tedeschi, Umberto; Salmaso, Beatrice; Buzzi, Gianluca; Parini, Dario; Prando, Daniela; Zuin, Matteo; Bergamo, Francesca; Zagonel, Vittorina; Porzionato, Andrea; Cavallin, Francesco; Camillo, Barbara Di; Cristoforo, Loretta Di; Bao, Quoc Riccardo; Pucciarelli, Salvatore; Bardini, Romeo; Spolverato, Gaya; Fassan, Matteo; Scarpa, Marco; Null, Null. - In: CANCER. - ISSN 0008-543X. - 130:13(2024), pp. 2272-2286. [10.1002/cncr.35297]
IMMUNOREACT 7: Regular aspirin use is associated with immune surveillance activation in colorectal cancer
Zizzo, Maurizio;
2024
Abstract
Background: Long-term daily use of aspirin reduces incidence and mortality due to colorectal cancer (CRC). This study aimed to analyze the effect of aspirin on the tumor microenvironment, systemic immunity, and on the healthy mucosa surrounding cancer. Methods: Patients with a diagnosis of CRC operated on from 2015 to 2019 were retrospectively analyzed (METACCRE cohort). Expression of mRNA of immune surveillance-related genes (PD-L1, CD80, CD86, HLA I, and HLA II) in CRC primary cells treated with aspirin were extracted from Gene Expression Omnibus–deposited public database (GSE76583). The experiment was replicated in cell lines. The mucosal immune microenvironment of a subgroup of patients participating in the IMMUNOREACT1 (ClinicalTrials.gov NCT04915326) project was analyzed with immunohistochemistry and flow cytometry. Results: In the METACCRE Cohort, 12% of 238 patients analyzed were aspirin users. Nodal metastasis was significantly less frequent (p =.008) and tumor-infiltrating lymphocyte infiltration was higher (p =.02) among aspirin users. In the CRC primary cells and selected cell lines, CD80 mRNA expression was increased following aspirin treatment (p =.001). In the healthy mucosa surrounding rectal cancer, the ratio of CD8/CD3 and epithelial cells expressing CD80 was higher in aspirin users (p =.027 and p =.034, respectively). Conclusions: These data suggested that regular aspirin use may have an active role in enhancing immunosurveillance against CRC.
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